Fig. 5.

Impact of Trim71 conditional knockout in the male germline. (A) Representative images of testis and epididymis from adult (P56) Trim71 conditional knockout (cKO) and control mice. Ruler shows 1 mm per division. (B) Representative images of Hematoxylin and Eosin (H&E)-stained cross-sections of seminiferous tubules and epididymis from P56 control and Trim71 cKO mice. (C-G) Representative images of immunofluorescence staining for the pan germ cell marker GCNA1 at P1 (C), the undifferentiated spermatogonial marker ZBTB16 and primitive spermatogonial marker GFRA1 at P10 (D) and P56 (E), and the undifferentiated spermatogonial marker ZBTB16 and differentiating marker STRA8 at P10 (F) and P56 (G) in cross-sections of testes from control and Trim71 cKO mice. (H) Quantification of total germ cell number (based on GCNA1⁺ cells) in seminiferous tubule cross-sections from control and Trim71 cKO mice at P1. (I,J) Quantification of undifferentiated spermatogonial number [based on ZBTB16⁺ cells/seminiferous tubule at P10 or number (#) of ZBTB16⁺ cells × seminiferous tubule diameter (µm) at P56], percentage of the population that is the most primitive subset (based on GFRA1⁺ among ZBTB16⁺ cells), and percentage of the population that has transitioned to a differentiating state (based on STRA8⁺ among ZBTB16⁺ cells) in cross-sections of testes from control and Trim71 cKO mice at prepubertal (P10) (I) and adult (P56) (J) age points. Data in H-J are mean±s.e.m. for n=3 different mice, dots represent average values of individual mice, **P<0.01 (Student's t-test). Scale bars: 20 μm.