Table 1.
Identified suitable positions for amber codon incorporation into FTN2 gene based on the defined rules. Nucleic acid (NA) for position +4 is in uppercase and bold. These data are obtained based on the X ray Chrystal structure of anti-TNFα Fab (PDB code 5WUV). Solvent accessibility for each position is calculated using CUPSAT and is valid for corresponding native amino acid and might be different upon substitution.
| Position | Nucleic acid at position +4 | AA context (position in structure free region) | Solvent accessibility for native AA [%] |
|---|---|---|---|
| SHC133 | tagAag | APSZKSTSGC (4/10) | 103.2 |
| KHC134 | tagAgc | APSSZSTSGC (5/10) | 82.6 |
| EHC89a | tagGat | RAZDT | 88.1 |
| GHC15 | tagGgc | QPZGS (3/5) | 57.2 |
| PHC41 | tagGgt | AZGKG (2/5) | 64.8 |
| NLC28 | tagGtt | SQZVGTN (3/7) | 76.5 |
| GLC41 | tagAag | KPZKAP (3/6) | 93.5 |
| SLC56 | tagGgt | YZGVPYR (2/7) | 98.9 |
| QLC100 | tagGgc | GZG (2/3) | 82.9 |
| TLC109 | tagGtt | RZVAAP (2/6) | 72.8 |
| ELC143 | tagGcg | YPRZA (4/5) | 93.9 |
a
this AA sequence builds a helix and Z is incorporated in the middle of the helix structure.