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[Preprint]. 2023 Nov 21:2023.11.20.567970. [Version 1] doi: 10.1101/2023.11.20.567970

Figure 3. Multiparametric nanoimaging using single bacterial probes indicates SasG-II binds a broader variety of ligands than SasG-I.

Figure 3.

(A) Height images (top) and adhesion images (bottom) of corneocytes recorded in PBS using a SasG-II, SasG-I, or EV (SasG[−]) cell probe. See also Figure S2–S4. (B) Histograms of adhesion forces registered on whole corneocytes (total of n = 9,590 curves for one representative SasG-II probe; n = 2,532 curves for one representative SasGI probe). The arrow at the top left of the histograms stands for the non-adhesive events. (C) Box plot comparing adhesion probabilities for SasG-II (n = 4 from 3 independent bacterial cultures), SasG-I (n = 4 from 2 independent bacterial cultures), SasG(−) (n = 4 from 2 independent bacterial cultures) or colloidal (n = 2) probes. For more data, see Figures S1, S2, and S3.