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. Author manuscript; available in PMC: 2023 Dec 3.
Published in final edited form as: Biochemistry. 2019 Sep 26;58(40):4112–4124. doi: 10.1021/acs.biochem.9b00593

Figure 3. Increased light scattering of deamidated human γS crystallin.

Figure 3.

(A) Relative Rayleigh light scattering traces of γS WT (black), N14D, (cyan) and N76D (dark red) during size-exclusion chromatography in-line with multiangle laser scattering. A high molecular weight peak (HMW, starred) was detected just after the void peak (arrow). (B) SDS-PAGE of WT (Lane 1), N14D (Lane 2) and N76D (Lane 3) γS-crystallins. (C) Table of relative light scattering intensity of peaks from B. (D) Relative turbidity of γS-crystallins during thermal-induced denaturation at 70 °C of WT (black), γS N14D (cyan), and γS N76D (red). Turbidity measured as change in O.D. at 405 nm, n=3 and SEM calculated via 2-way ANOVA with p < 0.05 (*). Curve fits were generated using nonlinear regression with PRISM 6. Initial rates of increase in turbidity were 0.03 min−1 for WT and 0.06 min−1 for N14D and N76D.