Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors

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(STAR Protocols 4, 102580; December 15, 2023)

In the originally published version of this article, early versions of Figures 7, 15, and 16 appeared instead of their final versions:

• •In Figure 7, pluripotency flow cytometry results were presented at the end of the sequence of panels depicting stage completion results, rather than at the beginning; the stage abbreviations were not spelled out and did not indicate the day of sample collection; and the panel for stage 5 completion displayed flow cytometry results for Glucagon and C-peptide instead of Nkx6.1 and C-Peptide.
• •In Figure 15, incorrect results were shown for markers of poly-hormonal cells.
• •In Figure 16, human Glucagon ELISA results were missing.

The figures have now been updated. The authors apologize for any confusion the initial error may have caused.

Figure 7. Example flow cytometry quantification of stage-specific markers (corrected)

Figure 7. Example flow cytometry quantification of stage-specific markers (original)

Figure 15. Expected stage-specific marker results (corrected)

Figure 15. Expected stage-specific marker results (original)

Figure 16. Expected glucose-stimulated hormone secretion function (corrected)

Figure 16. Expected glucose-stimulated hormone secretion function (original)