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. 2023 Nov 15;624(7990):192–200. doi: 10.1038/s41586-023-06739-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — 4,147 different endogenous c-terminally tagged yeast strains¹ were used for 4,147 independent pull-down experiments. Each strain therefore allows the purification of the individually tagged protein (bait) and its specific interactors. The original library of 4,159 strains was reduced by twelve strains to 4147, due to updates in ORF annotations (see Methods: Data processing and normalization).