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[Preprint]. 2023 Dec 1:2023.11.30.569407. [Version 1] doi: 10.1101/2023.11.30.569407

Figure 5: Chronic metabolic stress drives altered epigenetic trajectories and WNT pathway priming.

Figure 5:

(A) Chromatin accessibility deviation of TF motifs across age and diet conditions. (B) Epigenetic gene activity trajectories of HFD hepatocytes ordered by pseudotime progression. (C) Coverage plot of epigenetic accessibility at Axin2 gene locus across age and diet. (D) Accessibility changes of gene-linked peaks at 6-month timepoint, between actual observed HFD-vs-CD fold-changes (red) and GC-and-background-matched null distribution (gray). (E) Expression changes of genes at 6-month HFD-vs-CD (red) or 15-month tumor-vs-adjacent-normal (brown) comparisons. (F) Computationally-inferred network of TFs predicted to regulate MASLD-relevant gene programs. All p-values calculated using Mann-Whitney U test with Benjamini-Hochberg correction; effect size quantified through Cohen’s D.