FIGURE 6.

Northern analysis of RNA preparations made in acidic conditions to preserve aminoacyl linkages to tRNAs. Columns labeled “+” show separation of RNA treated with base to remove aminoacyl groups; those labeled “−” show separation of acidic RNA aminoacyl groups intact. Aminoacyl-tRNAs in the “−” columns are shifted upward relative to deacyl-tRNAs in the “+” column. Rows are identified as in Figure 5, and the modification enzymes that target each tRNA in the wild-type strain are indicated on the right (“+”, modified; “−” unmodified.)