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. 2023 Dec 18;12:RP88051. doi: 10.7554/eLife.88051

Figure 12. Reduced RET signaling accelerates ENS aging to cause pathology.

Figure 12.

(a) Hu immunostaining (green) LM-MP tissues from 16-week-old Ret+/CFP (Ret+/-) mouse shows mutually exclusive expression of Ret-CFP (cyan, white arrow) and MHCst (red, red arrow) MENs. Nuclei are stained with DAPI (blue). Scale bar = 10 µm. (b) Quantification of Ret-CFP+ neurons from 9- and 16-week-old Ret+/- +/- show age-associated loss of Ret-CFP+ neurons. Data represent mean ± S.E.M. Student’s t-test * p<0.05. (c) Quantification of MHCst+ MENs shows significant increase in their proportions in Ret +/-mice but not in Ret+/+ mice with age. Data represent mean ± S.E.M. One-way (ANOVA * p<0.05, ** p<0.01, *** p<0.001). (d) Measures of whole gut transit time (WGTT) in cohorts of Ret+/- and Ret+/+ mice MENs show significant slowing of whole gut transit of Ret+/- +/- not Ret+/+ mice with age. Data represent mean ± S.E.M. One-way ANOVA *=p < 0.05.