Abstract
Cell lines of tobacco (Nicotiana tabacum L. var Wisconsin 38) were obtained which are adapted to grow in media with varying concentrations of NaCl, up to 35 grams per liter (599 millimolar). Salt-adapted cells exhibited enhanced abilities to gain both fresh and dry weight in the presence of NaCl compared to cells which were growing in medium without NaCl (unadapted cells). Tolerance of unadapted cells and cells adapted to 10 grams per liter NaCl was influenced by the stage of growth, with the highest degree of tolerance exhibited by cells in the exponential phase. Cell osmotic potential and turgor varied through the growth cycle of unadapted cells and cells at all levels of adaptation, with maximum turgor occurring at approximately the onset of exponential fresh weight accumulation.
Adaptation to NaCl led to reduced cell expansion and fresh weight gain, while dry weight gain remained unaffected. This reduction in cell expansion was not due to failure of the cells to maintain turgor since cells adapted to NaCl underwent osmotic adjustment in excess of the change in water potential caused by the addition of NaCl to the medium. Tolerance of the adapted cells, as indicated by fresh or dry weight gain, did not increase proportionately with the increase in turgor. Adaptation of these glycophytic cells to NaCl appears to involve mechanisms which result in an altered relationship between turgor and cell expansion.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ben-Hayyim G., Kochba J. Aspects of Salt Tolerance in a NaCl-Selected Stable Cell Line of Citrus sinensis. Plant Physiol. 1983 Jul;72(3):685–690. doi: 10.1104/pp.72.3.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bolaños J. A., Longstreth D. J. Salinity Effects on Water Potential Components and Bulk Elastic Modulus of Alternanthera philoxeroides (Mart.) Griseb. Plant Physiol. 1984 Jun;75(2):281–284. doi: 10.1104/pp.75.2.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bressan R. A., Handa A. K., Handa S., Hasegawa P. M. Growth and water relations of cultured tomato cells after adjustment to low external water potentials. Plant Physiol. 1982 Nov;70(5):1303–1309. doi: 10.1104/pp.70.5.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Handa A. K., Bressan R. A., Handa S., Hasegawa P. M. Characteristics of cultured tomato cells after prolonged exposure to medium containing polyethylene glycol. Plant Physiol. 1982 Feb;69(2):514–521. doi: 10.1104/pp.69.2.514. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Handa S., Bressan R. A., Handa A. K., Carpita N. C., Hasegawa P. M. Solutes contributing to osmotic adjustment in cultured plant cells adapted to water stress. Plant Physiol. 1983 Nov;73(3):834–843. doi: 10.1104/pp.73.3.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heyser J. W., Nabors M. W. Osmotic Adjustment of Cultured Tobacco Cells (Nicotiana tabacum var. Samsum) Grown on Sodium Chloride. Plant Physiol. 1981 Apr;67(4):720–727. doi: 10.1104/pp.67.4.720. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsuda K., Riazi A. Stress-induced osmotic adjustment in growing regions of barley leaves. Plant Physiol. 1981 Sep;68(3):571–576. doi: 10.1104/pp.68.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michelena V. A., Boyer J. S. Complete turgor maintenance at low water potentials in the elongating region of maize leaves. Plant Physiol. 1982 May;69(5):1145–1149. doi: 10.1104/pp.69.5.1145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Volkenburgh E., Boyer J. S. Inhibitory effects of water deficit on maize leaf elongation. Plant Physiol. 1985 Jan;77(1):190–194. doi: 10.1104/pp.77.1.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watad A. E., Reinhold L., Lerner H. R. Comparison between a Stable NaCl-Selected Nicotiana Cell Line and the Wild Type : K, Na, and Proline Pools as a Function of Salinity. Plant Physiol. 1983 Nov;73(3):624–629. doi: 10.1104/pp.73.3.624. [DOI] [PMC free article] [PubMed] [Google Scholar]