Abstract
Callus cultures established from Phaseolus vulgaris seedlings were used to investigate hormonal influence on lectin biosynthesis. The plant tissue cultures were initiated using defined levels of both a cytokinin (kinetin) and an auxin (2,4-dichlorophenoxyacetic acid) and were then transferred to media containing different amounts of these hormones. The lectin content of each callus culture was determined using an enzyme immunoassay specific for the seed lectin of the P. vulgaris plant. The lectin biosynthesis was directly affected by the levels of auxin and cytokinin in the culture media and no lectin was detected in hormone-free medium. This enabled us to compose culture media yielding a maximal or minimal lectin content of the callus cultures, illustrating the ability to induce an enhancement or suppression of the in vitro lectin biosynthesis. The lectin level of callus tissue during the growth cycle of a culture was, furthermore, related to the cellular growth rate which might indicate an involvement of the lectin in cellular events during rapid cell division.
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- Etzler M. E., Borrebaeck C. Carbohydrate binding activity of a lectin-like glycoprotein from stems and leaves of Dolichos biflorus. Biochem Biophys Res Commun. 1980 Sep 16;96(1):92–97. doi: 10.1016/0006-291x(80)91185-7. [DOI] [PubMed] [Google Scholar]
- Etzler M. E., Kabat E. A. Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity from Dolichos biflorus. Biochemistry. 1970 Feb 17;9(4):869–877. doi: 10.1021/bi00806a022. [DOI] [PubMed] [Google Scholar]
- James D. W., Ghosh M., Etzler M. E. Production of a Lectin in Tissue Cultures of Dolichos biflorus. Plant Physiol. 1985 Mar;77(3):630–634. doi: 10.1104/pp.77.3.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lamb J. E., Shibata S., Goldstein I. J. Purification and Characterization of Griffonia simplicifolia Leaf Lectins. Plant Physiol. 1983 Apr;71(4):879–887. doi: 10.1104/pp.71.4.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meimeth T., Van K. T., Marcotte J. L., Trinn T. H., Clarke A. E. Distribution of Lectins in Tissues, Derived Callus, and Roots of Psophocarpus tetragonolobus (Winged Bean). Plant Physiol. 1982 Aug;70(2):579–584. doi: 10.1104/pp.70.2.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Read S. M., Northcote D. H. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem. 1981 Sep 1;116(1):53–64. doi: 10.1016/0003-2697(81)90321-3. [DOI] [PubMed] [Google Scholar]
- Roberts D. D., Goldstein I. J. Adenine binding sites of the lectin from lima beans (Phaseolus lunatus). J Biol Chem. 1983 Nov 25;258(22):13820–13824. [PubMed] [Google Scholar]
- Roberts D. M., Etzler M. E. Development and Distribution of a Lectin from the Stems and Leaves of Dolichos biflorus. Plant Physiol. 1984 Dec;76(4):879–884. doi: 10.1104/pp.76.4.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Talbot C. F., Etzler M. E. Development and Distribution of Dolichos biflorus Lectin as Measured by Radioimmunoassay. Plant Physiol. 1978 May;61(5):847–850. doi: 10.1104/pp.61.5.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Talbot C. F., Etzler M. E. Isolation and characterization of a protein from leaves and stems of Dolichos biflorus that cross reacts with antibodies to the seed lectin. Biochemistry. 1978 Apr 18;17(8):1474–1479. doi: 10.1021/bi00601a018. [DOI] [PubMed] [Google Scholar]
- Veliky I. A., Martin S. M. A fermenter for plant cell suspension cultures. Can J Microbiol. 1970 Apr;16(4):223–226. doi: 10.1139/m70-041. [DOI] [PubMed] [Google Scholar]