Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1985 Nov;79(3):829–832. doi: 10.1104/pp.79.3.829

Light Activation of NADP-Malate Dehydrogenase in Guard Cell Protoplasts from Vicia faba L. 1

Kiyoshi Gotow 1,2,3, Kiyoshi Tanaka 1,2,3, Noriaki Kondo 1,2,3, Koh Kobayashi 1,2,3, Kunihiko Syōno 1,2,3
PMCID: PMC1074978  PMID: 16664499

Abstract

Light-induced swelling of guard cell protoplasts (GCP) from Vicia faba was accompanied by increases in content of K+ and malate. DCMU inhibited the increase of K+ and malate, and consequently swelling.

Effect of light on the activity of selected enzymes that take part in malate formation was studied. When isolated GCP were illuminated, NADP-malate dehydrogenase (NADP-MDH) was activated, and the activity reached a maximum within 5 minutes. The enzyme activity underwent 5- to 6-fold increase in the light. Upon turning off the light, the enzyme was inactivated in 5 minutes NAD-MDH and phosphoenolpyruvate carboxylase (PEPC) were not influenced by light. The rapid light activation of NADP-MDH was inhibited by DCMU, suggesting that the enzyme was activated by reductants from the linear electron transport in chloroplasts. An enzyme localization study by differential centrifugation indicates that NADP-MDH is located in the chloroplasts, NAD-MDH in the cytosol and mitochondria, and PEPC in the cytosol. After light activation, the activity of NADP-MDH in guard cells was 10 times that in mesophyll cells on a chlorophyll basis. The physiological significance of light-dependent activation of NADP-MDH in guard cells is discussed in relation to stomatal movement.

Full text

PDF
829

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gotow K., Sakaki T., Kondo N., Kobayashi K., Syōno K. Light-Induced Alkalinization of the Suspending Medium of Guard Cell Protoplasts from Vicia faba L. Plant Physiol. 1985 Nov;79(3):825–828. doi: 10.1104/pp.79.3.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Madhavan S., Smith B. N. Localization of ribulose bisphosphate carboxylase in the guard cells by an indirect, immunofluorescence technique. Plant Physiol. 1982 Jan;69(1):273–277. doi: 10.1104/pp.69.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Martin G. E., Outlaw W. H., Anderson L. C., Jackson S. G. Photosynthetic electron transport in guard cells of diverse species. Plant Physiol. 1984 Jun;75(2):336–337. doi: 10.1104/pp.75.2.336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Maruyama H., Easterday R. L., Chang H. C., Lane M. D. The enzymatic carboxylation of phosphoenolpyruvate. I. Purification and properties of phosphoenolpyruvate carboxylase. J Biol Chem. 1966 May 25;241(10):2405–2412. [PubMed] [Google Scholar]
  5. Ogawa T., Grantz D., Boyer J., Govindjee Effects of Cations and Abscisic Acid on Chlorophyll a Fluorescence in Guard Cells of Vicia faba. Plant Physiol. 1982 May;69(5):1140–1144. doi: 10.1104/pp.69.5.1140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Outlaw W. H., Mayne B. C., Zenger V. E., Manchester J. Presence of Both Photosystems in Guard Cells of Vicia faba L: IMPLICATIONS FOR ENVIRONMENTAL SIGNAL PROCESSING. Plant Physiol. 1981 Jan;67(1):12–16. doi: 10.1104/pp.67.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Prins H. B., Harper J. R., Higinbotham N. Membrane potentials of vallisneria leaf cells and their relation to photosynthesis. Plant Physiol. 1980 Jan;65(1):1–5. doi: 10.1104/pp.65.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rao I. M., Anderson L. E. Light and stomatal metabolism : I. Possible involvement of light modulation of enzymes in stomatal movement. Plant Physiol. 1983 Mar;71(3):451–455. doi: 10.1104/pp.71.3.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sharkey T. D., Raschke K. Effect of Light Quality on Stomatal Opening in Leaves of Xanthium strumarium L. Plant Physiol. 1981 Nov;68(5):1170–1174. doi: 10.1104/pp.68.5.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Zeiger E., Armond P., Melis A. Fluorescence Properties of Guard Cell Chloroplasts: EVIDENCE FOR LINEAR ELECTRON TRANSPORT AND LIGHT-HARVESTING PIGMENTS OF PHOTOSYSTEMS I AND II. Plant Physiol. 1981 Jan;67(1):17–20. doi: 10.1104/pp.67.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Zeiger E., Hepler P. K. Light and stomatal function: blue light stimulates swelling of guard cell protoplasts. Science. 1977 May 20;196(4292):887–889. doi: 10.1126/science.196.4292.887. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES