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. 1985 Dec;79(4):928–934. doi: 10.1104/pp.79.4.928

An Investigation into the Role of Photosynthesis in Regulating ATP Levels and Rates of H+ Efflux in Isolated Meosphyll Cells 1

Alan W Bown 1, Freda Nicholls 1
PMCID: PMC1075001  PMID: 16664547

Abstract

Aerated and stirred 10-ml suspensions of mechanically isolated Asparagus sprengeri Regel mesophyll cells were used for simultaneous measurements of net H+ efflux and steady-state ATP levels.

Initial rates of medium acidification indicated values for H+ efflux in the light and dark of 0.66 and 0.77 nanomoles H+/106 cells per minute, respectively. When the medium pH was maintained at 6.5, with a pH-stat apparatus, rates of H+ efflux remained constant. Darkness or DCMU, however, stimulated H+ efflux by 100% or more. Darkness increased ATP levels by 33% and a switch from dark to light reduced ATP levels by 31%. In the absence of aeration, illumination prevented the accumulation of respiratory CO2 and the buffering capacity of the medium was about 50% less than that found in the nonilluminated nonaerated medium. As a result, rates of pH decline were similar even though the dark rate of H+ efflux was approximately 50% greater.

Proposals that photosynthesis stimulates H+ efflux are based on changes in the rate of pH decline. The present data indicate that photosynthesis inhibits H+ efflux and that changes in rates of pH decline should not be equated with changes in the rate of H+ efflux.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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