Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1985 Dec;79(4):1080–1085. doi: 10.1104/pp.79.4.1080

Auxin Regulation of a Proton Translocating ATPase in Pea Root Plasma Membrane Vesicles 1

Reinhard Gabathuler 1,2, Robert E Cleland 1
PMCID: PMC1075031  PMID: 16664534

Abstract

Pea root microsomal vesicles have been fractionated on a Dextran step gradient to give three fractions, each of which carries out ATP-dependent proton accumulation as measured by fluorescence quenching of quinacrine. The fraction at the 4/6% Dextran interface is enriched in plasma membrane, as determined by UDPG sterol glucosyltransferase and vanadate-inhibited ATPase. The vanadate-sensitive phosphohydrolase is not specific for ATP, has a Km of about 0.23 millimolar for MgATP, is only slightly affected by K+ or Cl− and is insensitive to auxin. Proton transport, on the other hand, is more specific for ATP, enhanced by anions (NO3− > Cl−) and has a Km of about 0.7 millimolar. Auxins decrease the Km to about 0.35 millimolar, with no significant effect on the Vmax, while antiauxins or weak acids have no such effect. It appears that auxin has the ability to alter the efficiency of the ATP-driven proton transport.

Full text

PDF
1080

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Choquette D., Hakim G., Filoteo A. G., Plishker G. A., Bostwick J. R., Penniston J. T. Regulation of plasma membrane Ca2+ ATPases by lipids of the phosphatidylinositol cycle. Biochem Biophys Res Commun. 1984 Dec 28;125(3):908–915. doi: 10.1016/0006-291x(84)91369-x. [DOI] [PubMed] [Google Scholar]
  2. Cross J. W., Briggs W. R. Auxin receptors of maize coleoptile membranes do not have ATPase activity. Plant Physiol. 1978 Apr;61(4):581–584. doi: 10.1104/pp.61.4.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hodges T. K., Leonard R. T. Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. Methods Enzymol. 1974;32:392–406. doi: 10.1016/0076-6879(74)32039-3. [DOI] [PubMed] [Google Scholar]
  4. Jacobs M., Taiz L. Vanadate inhibition of auxin-enhanced H secretion and elongation in pea epicotyls and oat coleoptiles. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7242–7246. doi: 10.1073/pnas.77.12.7242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Morré D. J., Gripshover B., Monroe A., Morré J. T. Phosphatidylinositol turnover in isolated soybean membranes stimulated by the synthetic growth hormone 2,4-dichlorophenoxyacetic acid. J Biol Chem. 1984 Dec 25;259(24):15364–15368. [PubMed] [Google Scholar]
  7. Mulkey T. J., Kuzmanoff K. M., Evans M. L. Promotion of growth and hydrogen ion efflux by auxin in roots of maize pretreated with ethylene biosynthesis inhibitors. Plant Physiol. 1982 Jul;70(1):186–188. doi: 10.1104/pp.70.1.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Navarro J., Toivio-Kinnucan M., Racker E. Effect of lipid composition on the calcium/adenosine 5'-triphosphate coupling ratio of the Ca2+-ATPase of sarcoplasmic reticulum. Biochemistry. 1984 Jan 3;23(1):130–135. doi: 10.1021/bi00296a021. [DOI] [PubMed] [Google Scholar]
  9. O'neill S. D., Spanswick R. M. Effects of vanadate on the plasma membrane ATPase of red beet and corn. Plant Physiol. 1984 Jul;75(3):586–591. doi: 10.1104/pp.75.3.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rayle D. L., Cleland R. Control of plant cell enlargement by hydrogen ions. Curr Top Dev Biol. 1977;11:187–214. doi: 10.1016/s0070-2153(08)60746-2. [DOI] [PubMed] [Google Scholar]
  11. Scarborough G. A., Addison R. On the subunit composition of the Neurospora plasma membrane H+-ATPase. J Biol Chem. 1984 Jul 25;259(14):9109–9114. [PubMed] [Google Scholar]
  12. Serrano R. Purification and reconstitution of the proton-pumping ATPase of fungal and plant plasma membranes. Arch Biochem Biophys. 1983 Nov;227(1):1–8. doi: 10.1016/0003-9861(83)90342-9. [DOI] [PubMed] [Google Scholar]
  13. Stout R. G., Cleland R. E. Evidence for a Cl-Stimulated MgATPase Proton Pump in Oat Root Membranes. Plant Physiol. 1982 Apr;69(4):798–803. doi: 10.1104/pp.69.4.798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sze H. Nigericin-stimulated ATPase activity in microsomal vesicles of tobacco callus. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5904–5908. doi: 10.1073/pnas.77.10.5904. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES