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. 1986 Jan;80(1):126–129. doi: 10.1104/pp.80.1.126

Localization of Carbamoylphosphate Synthetase and Aspartate Carbamoyltransferase in Chloroplasts

Hitoshi Shibata 1, Hideo Ochiai 1, Yoshihiro Sawa 1, Shoji Miyoshi 1
PMCID: PMC1075068  PMID: 16664566

Abstract

The localization of carbamoylphosphate synthetase (CPSase) and aspartate carbamoyltransferase (ACTase), the first two enzymes of the pyrimidine biosynthetic pathway, in chloroplasts was investigated. In dark-grown radish (Raphanus sativus) seedlings, light induced a prominent increase in CPSase activity, but had little effect on ACTase activity. Both enzymes were found in chloroplasts isolated from radish cotyledons and leaves of spinach (Spinacia oleracea), soybean (Glycine max), and corn (Zea mays). The higher activity of ACTase relative to CPSase is discussed in relation to the instability of carbamoylphosphate, the product of the CPSase, and to the control of pyrimidine synthesis. Based on these results, the function of CPSase and ACTase in chloroplasts is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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