Abstract
Eleven endogenous gibberellins (GAs) were identified by combined gas chromatography-mass spectrometry in purified extracts from shoots of field pennycress (Thlaspi arvense L.): GA1,9,12,15,19,20,24,29,44,51,53. Traces of GA8 and GA25 were tentatively indicated by combined gas chromatography-mass spectrometry-selected ion monitoring. Comparison of the total ion current traces indicated that GA19 and GA44 were most abundant, while GA12,15,20,24,29,53 occurred in lesser amounts. Only small amounts of GA1,9,51 were present. The levels of GA8 and GA25 were barely detectable. Consideration of hydroxylation patterns of the ent-gibberellane ring structure indicates two families of GAs: one with a C-13 hydroxyl group (GA1,8,19,20,29,44,53) and another whose members are either nonhydroxylated (GA9,12,15,24,25) or lack a C-13 hydroxyl group (GA51). This suggests that in field pennycress there are two parallel pathways for GA metabolism with an early branch point from GA12: an early C-13 hydroxylation pathway, leading ultimately to GA1 and GA8 and a C-13 deoxy pathway culminating in the formation of GA9 and GA51.
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Selected References
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- Jones M. G., Metzger J. D., Zeevaart J. A. Fractionation of gibberellins in plant extracts by reverse phase high performance liquid chromatography. Plant Physiol. 1980 Feb;65(2):218–221. doi: 10.1104/pp.65.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Metzger J. D. Role of Gibberellins in the Environmental Control of Stem Growth in Thlaspi arvense L. Plant Physiol. 1985 May;78(1):8–13. doi: 10.1104/pp.78.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Metzger J. D., Zeevaart J. A. Identification of six endogenous gibberellins in spinach shoots. Plant Physiol. 1980 Apr;65(4):623–626. doi: 10.1104/pp.65.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]