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. 1986 Apr;80(4):931–937. doi: 10.1104/pp.80.4.931

Reconstitution of the Light Harvesting Chlorophyll a/b Pigment-Protein Complex into Developing Chloroplast Membranes Using a Dialyzable Detergent 1

Sylvia C Darr 1,2, Charles J Arntzen 1,2
PMCID: PMC1075232  PMID: 16664744

Abstract

Conditions were developed to isolate the light-harvesting chlorophyll-protein complex serving photosystem II (LHC-II) using a dialyzable detergent, octylpolyoxyethylene. This LHC-II was successfully reconstituted into partially developed chloroplast thylakoids of Hordeum vulgare var Morex (barley) seedlings which were deficient in LHC-II. Functional association of LHC-II with the photosystem II (PSII) core complex was measured by two independent functional assays of PSII sensitization by LHC-II. A 3-fold excess of reconstituted LHC-II was required to equal the activity of LHC developing in vivo. We suggest that a linker component may be absent in the partially developed membranes which is required for specific association of the PSII core complex and LHC-II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argyroudi-Akoyunoglou J. H., Feleki Z., Akoyunoglou G. Formation of two chlorophyll-protein complexes during greening of etiolated bean leaves. Biochem Biophys Res Commun. 1971 Nov 5;45(3):606–614. doi: 10.1016/0006-291x(71)90460-8. [DOI] [PubMed] [Google Scholar]
  2. Armond P. A., Staehelin L. A., Arntzen C. J. Spatial relationship of photosystem I, photosystem II, and the light-harvesting complex in chloroplast membranes. J Cell Biol. 1977 May;73(2):400–418. doi: 10.1083/jcb.73.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bennett J. Regulation of photosynthesis by reversible phosphorylation of the light-harvesting chlorophyll a/b protein. Biochem J. 1983 Apr 15;212(1):1–13. doi: 10.1042/bj2120001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boardman N. K., Thorne S. W. Sensitive fluorescence method for the determination of chlorophyll a-chlorophyll b ratios. Biochim Biophys Acta. 1971 Nov 2;253(1):222–231. doi: 10.1016/0005-2728(71)90248-9. [DOI] [PubMed] [Google Scholar]
  5. Burke J. J., Ditto C. L., Arntzen C. J. Involvement of the light-harvesting complex in cation regulation of excitation energy distribution in chloroplasts. Arch Biochem Biophys. 1978 Apr 15;187(1):252–263. doi: 10.1016/0003-9861(78)90031-0. [DOI] [PubMed] [Google Scholar]
  6. Darr S., Souza Machado V., Arntzen C. J. Uniparental inheritance of a chloroplast photosystem II polypeptide controlling herbicide binding. Biochim Biophys Acta. 1981 Jan 14;634(1):219–228. doi: 10.1016/0005-2728(81)90140-7. [DOI] [PubMed] [Google Scholar]
  7. Day D. A., Ryrie I. J., Fuad N. Investigations of the role of the main light-harvesting chlorophyll-protein complex in thylakoid membranes. Reconstitution of depleted membranes from intermittent-light-grown plants with the isolated complex. J Cell Biol. 1984 Jan;98(1):163–172. doi: 10.1083/jcb.98.1.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Glazer A. N. Comparative biochemistry of photosynthetic light-harvesting systems. Annu Rev Biochem. 1983;52:125–157. doi: 10.1146/annurev.bi.52.070183.001013. [DOI] [PubMed] [Google Scholar]
  9. Kyle D. J., Staehelin L. A., Arntzen C. J. Lateral mobility of the light-harvesting complex in chloroplast membranes controls excitation energy distribution in higher plants. Arch Biochem Biophys. 1983 Apr 15;222(2):527–541. doi: 10.1016/0003-9861(83)90551-9. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. McCourt P., Browse J., Watson J., Arntzen C. J., Somerville C. R. Analysis of Photosynthetic Antenna Function in a Mutant of Arabidopsis thaliana (L.) Lacking trans-Hexadecenoic Acid. Plant Physiol. 1985 Aug;78(4):853–858. doi: 10.1104/pp.78.4.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McDonnel A., Staehelin L. A. Adhesion between liposomes mediated by the chlorophyll a/b light-harvesting complex isolated from chloroplast membranes. J Cell Biol. 1980 Jan;84(1):40–56. doi: 10.1083/jcb.84.1.40. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mullet J. E., Arntzen C. J. Simulation of grana stacking in a model membrane system. Mediation by a purified light-harvesting pigment-protein complex from chloroplasts. Biochim Biophys Acta. 1980 Jan 4;589(1):100–117. doi: 10.1016/0005-2728(80)90135-8. [DOI] [PubMed] [Google Scholar]
  14. Mullet J. E., Burke J. J., Arntzen C. J. A developmental study of photosystem I peripheral chlorophyll proteins. Plant Physiol. 1980 May;65(5):823–827. doi: 10.1104/pp.65.5.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mullet J. E., Burke J. J., Arntzen C. J. Chlorophyll proteins of photosystem I. Plant Physiol. 1980 May;65(5):814–822. doi: 10.1104/pp.65.5.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ryrie I. J., Anderson J. M., Goodchild D. J. The role of the light-harvesting chlorophyll a/b protein complex in chloroplast membrane stacking. Cation-induced aggregation of reconstituted proteoliposomes. Eur J Biochem. 1980 Jun;107(2):345–354. doi: 10.1111/j.1432-1033.1980.tb06035.x. [DOI] [PubMed] [Google Scholar]
  17. Schmidt G. W., Bartlett S. G., Grossman A. R., Cashmore A. R., Chua N. H. Biosynthetic pathways of two polypeptide subunits of the light-harvesting chlorophyll a/b protein complex. J Cell Biol. 1981 Nov;91(2 Pt 1):468–478. doi: 10.1083/jcb.91.2.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sprague S. G., Camm E. L., Green B. R., Staehelin L. A. Reconstitution of light-harvesting complexes and photosystem II cores into galactolipid and phospholipid liposomes. J Cell Biol. 1985 Feb;100(2):552–557. doi: 10.1083/jcb.100.2.552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Steinback K. E., Burke J. J., Arntzen C. J. Evidence for the role of surface-exposed segments of the light-harvesting complex in cation-mediated control of chloroplast structure and function. Arch Biochem Biophys. 1979 Jul;195(2):546–557. doi: 10.1016/0003-9861(79)90381-3. [DOI] [PubMed] [Google Scholar]
  20. Thornber J. P., Highkin H. R. Composition of the photosynthetic apparatus of normal barley leaves and a mutant lacking chlorophyll b. Eur J Biochem. 1974 Jan 3;41(1):109–116. doi: 10.1111/j.1432-1033.1974.tb03250.x. [DOI] [PubMed] [Google Scholar]

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