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. 1986 Apr;80(4):1051–1054. doi: 10.1104/pp.80.4.1051

Expression of Two Different Glutamine Synthetase Polypeptides during Root Development in Phaseolus vulgaris L. 1

José Luis Ortega 1, Francisco Campos 1, Federico Sánchez 1, Miguel Lara 1
PMCID: PMC1075256  PMID: 16664719

Abstract

Immunoprecipitation and two-dimensional gel electrophoresis analysis of the glutamine synthetase (GS) polypeptides (α and β) during Phaseolus vulgaris root development shows that the α polypeptide is the main component of the enzyme in the embryo and in up to 5 day old roots. From 5 days on, the β polypeptide becomes the root predominant GS monomer. The α/β ratio of the in vitro translated GS polypeptides from the total polysomal RNA isolated at different root ages correlates with the α/β ratio observed in the root extracts. These results suggest that the two root GS polypeptides are encoded by different mRNA species in Phaseolus vulgaris.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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