Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1986 May;81(1):293–296. doi: 10.1104/pp.81.1.293

Phosphoserine Aminotransferase in Soybean Root Nodules 1

Demonstration and Localization

Paul H S Reynolds 1,2,2, Dale G Blevins 1,2
PMCID: PMC1075322  PMID: 16664792

Abstract

Phosphoserine aminotransferase activity was detected in the plant and bacteroid fractions from soybean (Glycine max) root nodules. Both total and specific activities increased in the plant fraction during nodule development. Serine-pyruvate aminotransferase activity was not detectable in the plant or bacteroid fractions of these nodules. Sucrose density gradient fractionation indicated a proplastid localization for phosphoserine aminotransferase. The data presented support a role for this enzyme in carbon supply to purine biosynthesis in the pathway of ureide biogenesis in soybean nodules.

Full text

PDF
293

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boland M. J., Schubert K. R. Phosphoglycerate dehydrogenase from soybean nodules : partial purification and some kinetic properties. Plant Physiol. 1983 Mar;71(3):658–661. doi: 10.1104/pp.71.3.658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Cheung G. P., Rosenblum I. Y., Sallach H. J. Comparative studies of enzymes related to serine metabolism in higher plants. Plant Physiol. 1968 Nov;43(11):1813–1820. doi: 10.1104/pp.43.11.1813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hanks J. F., Tolbert N. E., Schubert K. R. Localization of enzymes of ureide biosynthesis in peroxisomes and microsomes of nodules. Plant Physiol. 1981 Jul;68(1):65–69. doi: 10.1104/pp.68.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Neal M. W., Florini J. R. A rapid method for desalting small volumes of solution. Anal Biochem. 1973 Sep;55(1):328–330. doi: 10.1016/0003-2697(73)90325-4. [DOI] [PubMed] [Google Scholar]
  6. RABSON R., TOLBERTNE, KEARNEY P. C. Formation of serine and glyceric acid by the glycolate pathway. Arch Biochem Biophys. 1962 Jul;98:154–163. doi: 10.1016/0003-9861(62)90161-3. [DOI] [PubMed] [Google Scholar]
  7. Reynolds P. H., Boland M. J., Blevins D. G., Schubert K. R., Randall D. D. Enzymes of amide and ureide biogenesis in developing soybean nodules. Plant Physiol. 1982 Jun;69(6):1334–1338. doi: 10.1104/pp.69.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Walsh D. A., Sallach H. J. Comparative studies on the pathways for serine biosynthesis in animal tissues. J Biol Chem. 1966 Sep 10;241(17):4068–4076. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES