Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1986 Jul;81(3):737–741. doi: 10.1104/pp.81.3.737

Effects of Nitrogen Dioxide and Nitrate Nutrition on Nodulation, Nitrogenase Activity, Growth, and Nitrogen Content of Bean 1

Hari S Srivastava 1,2,2, Douglas P Ormrod 1,2
PMCID: PMC1075418  PMID: 16664894

Abstract

The influence of nutrient nitrate level (0-20 millimolar) on the effects of NO2 (0-0.5 parts per million) on nodulation and in vivo acetylene reduction activity of the roots and on growth and nitrate and Kjeldahl N concentration in shoots was studied in bean (Phaseolus vulgaris L. cv Kinghorn Wax) plants. Exposing 8-day old seedlings for 6 hours each day, for 15 days, to 0.02 to 0.5 parts per million NO2 decreased total nodule weight at 0 and 1 millimolar nitrate, and nitrogenase (acetylene reduction) activity at all concentrations of nitrate. The pollutant had little effect on root fresh or dry weights. Shoot growth was inhibited by NO2. The NO2 exposure increased nitrate concentration in roots only at 20 millimolar nutrient nitrate. Exposure to NO2 markedly increased Kjeldahl N concentration in roots but generally decreased that in shoots. The experiments demonstrated that nutrient N level and NO2 concentration act jointly in affecting nodulation and N fixing capability, plant growth and composition, and root/shoot relationships of bean plants.

Full text

PDF
737

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Johnson H. S., Hume D. J. Comparisons of nitrogen fixation estimates in soybeans by nodule weight, leghemoglobin content, and acetylene reduction. Can J Microbiol. 1973 Sep;19(9):1165–1168. doi: 10.1139/m73-186. [DOI] [PubMed] [Google Scholar]
  2. Kennedy I. R., Rigaud J., Trinchant J. C. Nitrate reductase from bacteroides of Rhizobium japonicum: enzyme characteristics and possible interaction with nitrogen fixation. Biochim Biophys Acta. 1975 Jul 27;397(1):24–35. doi: 10.1016/0005-2744(75)90175-8. [DOI] [PubMed] [Google Scholar]
  3. Rigaud J., Puppo A. Effect of nitrite upon leghemoglobin and interaction with nitrogen fixation. Biochim Biophys Acta. 1977 May 26;497(3):702–706. doi: 10.1016/0304-4165(77)90291-4. [DOI] [PubMed] [Google Scholar]
  4. Rogers H. H., Campbell J. C., Volk R. J. Nitrogen-15 Dioxide Uptake and Incorporation by Phaseolus vulgaris (L.). Science. 1979 Oct 19;206(4416):333–335. doi: 10.1126/science.206.4416.333. [DOI] [PubMed] [Google Scholar]
  5. Srivastava H. S., Ormrod D. P. Effects of nitrogen dioxide and nitrate nutrition on growth and nitrate assimilation in bean leaves. Plant Physiol. 1984 Oct;76(2):418–423. doi: 10.1104/pp.76.2.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Streeter J. G. Nitrate inhibition of legume nodule growth and activity : I. Long term studies with a continuous supply of nitrate. Plant Physiol. 1985 Feb;77(2):321–324. doi: 10.1104/pp.77.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Streeter J. G. Synthesis and accumulation of nitrite in soybean nodules supplied with nitrate. Plant Physiol. 1982 Jun;69(6):1429–1434. doi: 10.1104/pp.69.6.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Taylor O. C., Eaton F. M. Suppression of plant growth by nitrogen dioxide. Plant Physiol. 1966 Jan;41(1):132–135. doi: 10.1104/pp.41.1.132. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES