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. 1986 Jul;81(3):788–791. doi: 10.1104/pp.81.3.788

Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Changing Partial Pressure of O2 and Light in Phaseolus vulgaris1

Thomas D Sharkey 1,2, Jeffrey R Seemann 1,2, Joseph A Berry 1,2
PMCID: PMC1075427  PMID: 16664903

Abstract

The regulation of ribulose-1,5-bisphosphate (RuBP) carboxylase (rubisco) activity in Phaseolus vulgaris was studied under moderate CO2 and high light, conditions in which photosynthesis in C3 plants can be insensitive to changes in O2 partial pressure. Steady state RuBP concentrations were higher, the calculated rate of RuBP use was lower and the activation state of rubisco was lower in low O2 relative to values observed in normal O2. It is suggested that the reduced activity of rubisco observed here is related to feedback effects which occur when the rate of net CO2 assimilation approaches the maximum capacity for starch and sucrose synthesis (triose phosphate utilization). The activation state of rubisco was independent of O2 partial pressure when light or CO2 was limiting for photosynthesis. Reduced activity of rubisco was also observed at limiting light. However, in this species light dependent changes in the concentration of an inhibitor of rubisco controlled the apparent Vmax of rubisco in low light while changes in the CO2-Mg2+ dependent activation of rubisco controlled the apparent Vmax in high light.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Enser U., Heber U. Metabolic regulation by pH gradients. Inhibition of photosynthesis by indirect proton transfer across the chloroplast envelope. Biochim Biophys Acta. 1980 Oct 3;592(3):577–591. doi: 10.1016/0005-2728(80)90102-4. [DOI] [PubMed] [Google Scholar]
  2. Evans J. R., Seemann J. R. Differences between Wheat Genotypes in Specific Activity of Ribulose-1,5-bisphosphate Carboxylase and the Relationship to Photosynthesis. Plant Physiol. 1984 Apr;74(4):759–765. doi: 10.1104/pp.74.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Harris G. C., Cheesbrough J. K., Walker D. A. Effects of mannose on photosynthetic gas exchange in spinach leaf discs. Plant Physiol. 1983 Jan;71(1):108–111. doi: 10.1104/pp.71.1.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Laing W. A. Regulation of Soybean Net Photosynthetic CO(2) Fixation by the Interaction of CO(2), O(2), and Ribulose 1,5-Diphosphate Carboxylase. Plant Physiol. 1974 Nov;54(5):678–685. doi: 10.1104/pp.54.5.678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lorimer G. H., Badger M. R., Andrews T. J. The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions. Equilibria, kinetics, a suggested mechanism, and physiological implications. Biochemistry. 1976 Feb 10;15(3):529–536. doi: 10.1021/bi00648a012. [DOI] [PubMed] [Google Scholar]
  6. Miziorko H. M., Lorimer G. H. Ribulose-1,5-bisphosphate carboxylase-oxygenase. Annu Rev Biochem. 1983;52:507–535. doi: 10.1146/annurev.bi.52.070183.002451. [DOI] [PubMed] [Google Scholar]
  7. Mott K. A., Jensen R. G., O'leary J. W., Berry J. A. Photosynthesis and Ribulose 1,5-Bisphosphate Concentrations in Intact Leaves of Xanthium strumarium L. Plant Physiol. 1984 Dec;76(4):968–971. doi: 10.1104/pp.76.4.968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Perchorowicz J. T., Raynes D. A., Jensen R. G. Light limitation of photosynthesis and activation of ribulose bisphosphate carboxylase in wheat seedlings. Proc Natl Acad Sci U S A. 1981 May;78(5):2985–2989. doi: 10.1073/pnas.78.5.2985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Seemann J. R., Badger M. R., Berry J. A. Variations in the Specific Activity of Ribulose-1,5-bisphosphate Carboxylase between Species Utilizing Differing Photosynthetic Pathways. Plant Physiol. 1984 Apr;74(4):791–794. doi: 10.1104/pp.74.4.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Seemann J. R., Berry J. A., Freas S. M., Krump M. A. Regulation of ribulose bisphosphate carboxylase activity in vivo by a light-modulated inhibitor of catalysis. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8024–8028. doi: 10.1073/pnas.82.23.8024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Servaites J. C. Binding of a Phosphorylated Inhibitor to Ribulose Bisphosphate Carboxylase/Oxygenase during the Night. Plant Physiol. 1985 Aug;78(4):839–843. doi: 10.1104/pp.78.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sharkey T. D. O(2)-insensitive photosynthesis in c(3) plants : its occurrence and a possible explanation. Plant Physiol. 1985 May;78(1):71–75. doi: 10.1104/pp.78.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Taylor S. E., Terry N. Limiting Factors in Photosynthesis: V. Photochemical Energy Supply Colimits Photosynthesis at Low Values of Intercellular CO(2) Concentration. Plant Physiol. 1984 May;75(1):82–86. doi: 10.1104/pp.75.1.82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vu J. C., Allen L. H., Bowes G. Dark/Light modulation of ribulose bisphosphate carboxylase activity in plants from different photosynthetic categories. Plant Physiol. 1984 Nov;76(3):843–845. doi: 10.1104/pp.76.3.843. [DOI] [PMC free article] [PubMed] [Google Scholar]

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