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. 1986 Jul;81(3):817–822. doi: 10.1104/pp.81.3.817

Uptake and Processing of the Precursor to the Small Subunit of Ribulose 1,5-Bisphosphate Carboxylase by Leucoplasts from the Endosperm of Developing Castor Oil Seeds 1

Sheila A Boyle 1, Sean M Hemmingsen 1, David T Dennis 1
PMCID: PMC1075433  PMID: 16664909

Abstract

Intact leucoplasts from the endosperm of developing castor oil seed were isolated by Percoll density gradient centrifugation. The precursor to the small subunit of ribulose 1,5-bisphosphate carboxylase from pea was synthesized in vitro from hybrid-selected mRNA. Leucoplasts imported this precursor by an ATP-requiring mechanism similar to that described in chloroplasts (AR Grossman et al. 1980 Nature 285: 625-628). The small subunit precursor was processed to a molecular weight that was identical with that of the mature pea small subunit. These results show that leucoplasts, though specialized for fatty acid biosynthesis and not photosynthesis, have a mechanism of protein import similar to that of chloroplasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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