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. 1986 Aug;81(4):1017–1021. doi: 10.1104/pp.81.4.1017

Abscisic Acid Accumulation by in Situ and Isolated Guard Cells of Pisum sativum L. and Vicia faba L. in Relation to Water Stress 1

Katrina Cornish 1,2, Jan A D Zeevaart 1
PMCID: PMC1075478  PMID: 16664936

Abstract

Isolated guard cells, prepared by sonication of epidermal peels, were used to investigate the endogenous level of abscisic acid (ABA) in the guard cells of turgid and stressed leaves of Vicia faba L. and the argenteum (arg) mutant of Pisum sativum L. The guard cells of V. faba and arg were found to contain 18 and 8 times more ABA, respectively, when isolated from stressed leaves than from turgid leaves. Isolated guard cells of V. faba were also directly stressed with the osmoticum Aquacide III. These guard cells were capable of producing stress-induced ABA to at least 3 times their ABA level when non-stressed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackerson R. C. Synthesis and movement of abscisic Acid in water-stressed cotton leaves. Plant Physiol. 1982 Mar;69(3):609–613. doi: 10.1104/pp.69.3.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cornish K., Zeevaart J. A. Abscisic Acid Accumulation by Roots of Xanthium strumarium L. and Lycopersicon esculentum Mill. in Relation to Water Stress. Plant Physiol. 1985 Nov;79(3):653–658. doi: 10.1104/pp.79.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cornish K., Zeevaart J. A. Abscisic Acid Metabolism in Relation to Water Stress and Leaf Age in Xanthium strumarium. Plant Physiol. 1984 Dec;76(4):1029–1035. doi: 10.1104/pp.76.4.1029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cornish K., Zeevaart J. A. Movement of Abscisic Acid into the Apoplast in Response to Water Stress in Xanthium strumarium L. Plant Physiol. 1985 Jul;78(3):623–626. doi: 10.1104/pp.78.3.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Creelman R. A., Zeevaart J. A. Abscisic Acid accumulation in spinach leaf slices in the presence of penetrating and nonpenetrating solutes. Plant Physiol. 1985 Jan;77(1):25–28. doi: 10.1104/pp.77.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grantz D. A., Graan T., Boyer J. S. Chloroplast Function in Guard Cells of Vicia faba L. : Measurement of the Electrochromic Absorbance Change at 518 nm. Plant Physiol. 1985 Apr;77(4):956–962. doi: 10.1104/pp.77.4.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grantz D. A., Ho T. H., Uknes S. J., Cheeseman J. M., Boyer J. S. Metabolism of Abscisic Acid in Guard Cells of Vicia faba L. and Commelina communis L. Plant Physiol. 1985 May;78(1):51–56. doi: 10.1104/pp.78.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ogawa T., Grantz D., Boyer J., Govindjee Effects of Cations and Abscisic Acid on Chlorophyll a Fluorescence in Guard Cells of Vicia faba. Plant Physiol. 1982 May;69(5):1140–1144. doi: 10.1104/pp.69.5.1140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Zeevaart J. A. Metabolism of Abscisic Acid and Its Regulation in Xanthium Leaves during and after Water Stress. Plant Physiol. 1983 Mar;71(3):477–481. doi: 10.1104/pp.71.3.477. [DOI] [PMC free article] [PubMed] [Google Scholar]

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