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. 1986 Aug;81(4):1123–1129. doi: 10.1104/pp.81.4.1123

Limitation of Photosynthesis by Carbon Metabolism 1

II. O2-Insensitive CO2 Uptake Results from Limitation Of Triose Phosphate Utilization

Thomas D Sharkey 1,2,2, Mark Stitt 1,2, Dieter Heineke 1,2, Richard Gerhardt 1,2, Klaus Raschke 1,2, Hans W Heldt 1,2
PMCID: PMC1075496  PMID: 16664954

Abstract

The occurrence of O2-insensitive photosynthesis at high quantum flux and moderate temperature in Spinacia oleracea was characterized by analytical gas exchange measurements on intact leaves. In addition photosynthetic metabolite pools were measured in leaves which had been rapidly frozen under defined gas conditions. Upon switching to low O2 in O2-insensitive conditions the ATP/ADP ratio fell dramatically within one minute. The P-glycerate pool increased over the same time. Ribulose bisphosphate initially declined, then increased and exceeded the pool size measured in air. The pools of hexose monophosphates and UDPglucose were higher at a partial pressure of O2 of 21 millibars than at 210 millibars. These results are consistent with the hypothesis that the rate of sucrose synthesis limited the overall rate of assimilation under O2-insensitive conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Foyer C., Walker D., Spencer C., Mann B. Observations on the phosphate status and intracellular pH of intact cells, protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance. Biochem J. 1982 Feb 15;202(2):429–434. doi: 10.1042/bj2020429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gerhardt R., Heldt H. W. Measurement of subcellular metabolite levels in leaves by fractionation of freeze-stopped material in nonaqueous media. Plant Physiol. 1984 Jul;75(3):542–547. doi: 10.1104/pp.75.3.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Giersch C., Heber U., Kobayashi Y., Inoue Y., Shibata K., Heldt H. W. Energy charge, phosphorylation potential and proton motive force in chloroplasts. Biochim Biophys Acta. 1980 Mar 7;590(1):59–73. doi: 10.1016/0005-2728(80)90146-2. [DOI] [PubMed] [Google Scholar]
  4. Harris G. C., Cheesbrough J. K., Walker D. A. Measurement of CO(2) and H(2)O Vapor Exchange in Spinach Leaf Discs : Effects of Orthophosphate. Plant Physiol. 1983 Jan;71(1):102–107. doi: 10.1104/pp.71.1.102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Rebeille F., Bligny R., Martin J. B., Douce R. Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch Biochem Biophys. 1983 Aug;225(1):143–148. doi: 10.1016/0003-9861(83)90017-6. [DOI] [PubMed] [Google Scholar]
  6. Sharkey T. D. O(2)-insensitive photosynthesis in c(3) plants : its occurrence and a possible explanation. Plant Physiol. 1985 May;78(1):71–75. doi: 10.1104/pp.78.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sivak M. N., Dietz K. J., Heber U., Walker D. A. The relationship between light scattering and chlorophyll a fluorescence during oscillations in photosynthetic carbon assimilation. Arch Biochem Biophys. 1985 Mar;237(2):513–519. doi: 10.1016/0003-9861(85)90304-2. [DOI] [PubMed] [Google Scholar]
  8. Stitt M. Limitation of Photosynthesis by Carbon Metabolism : I. Evidence for Excess Electron Transport Capacity in Leaves Carrying Out Photosynthesis in Saturating Light and CO(2). Plant Physiol. 1986 Aug;81(4):1115–1122. doi: 10.1104/pp.81.4.1115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Usuda H., Edwards G. E. Localization of glycerate kinase and some enzymes for sucrose synthesis in c(3) and c(4) plants. Plant Physiol. 1980 May;65(5):1017–1022. doi: 10.1104/pp.65.5.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]

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