Abstract
Catalase activity in grapevine (Vitis vinifera L.) buds cv. `Perlette.' increased to a maximum in October and thereafter decreased within 3 months to less than half its maximal rate. The decrease in catalase activity coincided with the decline in temperature during winter. The rate of sprouting of buds forced at 23°C was negatively related to the activity of catalase. Artificial chilling of grapevine canes at 5°C resulted in a 25% decrease of catalase activity in the buds after 3 days and 31% after 17 days. The activity of catalase increased to the control level only 96 hours after removing canes from 5°C to room temperature. Efficient buddormancy breaking agents, such as thiourea and cyanamide decreased catalase activity to 64 and 50% of the controls respectively, while the activity of peroxidase remained the same under those conditions. A less efficient dormancy breaking agent dinitro-ortho-cresol, did not decrease catalase activity.
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- AMBERGER A. [On the inhibition of plant catalase by cyanamide]. Hoppe Seylers Z Physiol Chem. 1961 Sep 20;325:183–188. doi: 10.1515/bchm2.1961.325.1.183. [DOI] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
- Hendricks S. B., Taylorson R. B. Breaking of seed dormancy by catalase inhibition. Proc Natl Acad Sci U S A. 1975 Jan;72(1):306–309. doi: 10.1073/pnas.72.1.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Omran R. G. Peroxide Levels and the Activities of Catalase, Peroxidase, and Indoleacetic Acid Oxidase during and after Chilling Cucumber Seedlings. Plant Physiol. 1980 Feb;65(2):407–408. doi: 10.1104/pp.65.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Patterson B. D., Payne L. A., Chen Y. Z., Graham D. An inhibitor of catalase induced by cold in chilling-sensitive plants. Plant Physiol. 1984 Dec;76(4):1014–1018. doi: 10.1104/pp.76.4.1014. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rorth M., Jensen P. K. Determination of catalase activity by means of the Clark oxygen electrode. Biochim Biophys Acta. 1967 May 16;139(1):171–173. doi: 10.1016/0005-2744(67)90124-6. [DOI] [PubMed] [Google Scholar]