Abstract
Embryogenesis in a wild carrot cell line, W001C, can start and progress up to the first morphogenetic stage (the globular-stage embryo) in 2,4-dichloropenoxyacetic acid (2,4-D). To clarify the quantitative effect of 2,4-D on this cell line, morphological and biochemical criteria have been used to monitor embryogenesis in the presence of increasing concentrations of 2,4-D. The biochemical criteria are the ability to inactivate cycloheximide and the expression of an embryogenic polypeptide E1. The results show that 2,4-D can affect embryogenesis in a quantitative manner but never fully suppresses embryogenesis unless it is coupled with high cell density.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Pitto L., Schiavo F. L., Terzi M. alpha-Amanitin resistance is developmentally regulated in carrot. Proc Natl Acad Sci U S A. 1985 May;82(9):2799–2803. doi: 10.1073/pnas.82.9.2799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sung Z. R. Cycloheximide resistance in carrot culture: a differentiated function. Plant Physiol. 1981 Jul;68(1):261–264. doi: 10.1104/pp.68.1.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sung Z. R., Okimoto R. Coordinate gene expression during somatic embryogenesis in carrots. Proc Natl Acad Sci U S A. 1983 May;80(9):2661–2665. doi: 10.1073/pnas.80.9.2661. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sung Z. R., Okimoto R. Embryonic proteins in somatic embryos of carrot. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3683–3687. doi: 10.1073/pnas.78.6.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sung Z. R. Turbidimetric measurement of plant cell culture growth. Plant Physiol. 1976 Mar;57(3):460–462. doi: 10.1104/pp.57.3.460. [DOI] [PMC free article] [PubMed] [Google Scholar]