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. 1992 Sep;100(1):131–137. doi: 10.1104/pp.100.1.131

1-Aminocyclopropane-1-Carboxylic Acid Transported from Roots to Shoots Promotes Leaf Abscission in Cleopatra Mandarin (Citrus reshni Hort. ex Tan.) Seedlings Rehydrated after Water Stress 1

Darius Tudela 1, Eduardo Primo-Millo 1
PMCID: PMC1075527  PMID: 16652935

Abstract

The effect of water stress and subsequent rehydration on 1-aminocyclopropane-1-carboxylic acid (ACC) content, ACC synthase activity, ethylene production, and leaf abscission was studied in Cleopatra mandarin (Citrus reshni Hort. ex Tan.) seedlings. Leaf abscission occurred when drought-stressed plants were allowed to rehydrate, whereas no abscission was observed in plants under water stress conditions. In roots of water-stressed plants, a high ACC accumulation and an increase in ACC synthase activity were observed. Neither increase in ACC content nor significant ethylene production were detected in leaves of water-stressed plants. After rehydration, a sharp rise in ACC content and ethylene production was observed in leaves of water-stressed plants. Content of ACC in xylem fluid was 10-fold higher in plants rehydrated for 2 h after water stress than in nonstressed plants. Leaf abscission induced by rehydration after drought stress was inhibited when roots or shoots were treated before water stress with aminooxyacetic acid (AOA, inhibitor of ACC synthase) or cobalt ion (inhibitor of ethylene-forming enzyme), respectively. However, AOA treatments to shoots did not suppress leaf abscission. The data indicate that water stress promotes ACC synthesis in roots of Cleopatra mandarin seedlings. Rehydration of plants results in ACC transport to the shoots, where it is oxidized to ethylene. Subsequently, this ethylene induces leaf abscission.

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Selected References

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  1. Aharoni N. Relationship between Leaf Water Status and Endogenous Ethylene in Detached Leaves. Plant Physiol. 1978 Apr;61(4):658–662. doi: 10.1104/pp.61.4.658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ben-Yehoshua S., Aloni B. Effect of Water Stress on Ethylene Production by Detached Leaves of Valencia Orange (Citrus sinensis Osbeck). Plant Physiol. 1974 Jun;53(6):863–865. doi: 10.1104/pp.53.6.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradford K. J., Hsiao T. C., Yang S. F. Inhibition of ethylene synthesis in tomato plants subjected to anaerobic root stress. Plant Physiol. 1982 Nov;70(5):1503–1507. doi: 10.1104/pp.70.5.1503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford K. J., Yang S. F. Xylem Transport of 1-Aminocyclopropane-1-carboxylic Acid, an Ethylene Precursor, in Waterlogged Tomato Plants. Plant Physiol. 1980 Feb;65(2):322–326. doi: 10.1104/pp.65.2.322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  6. Guinn G. Water deficit and ethylene evolution by young cotton bolls. Plant Physiol. 1976 Mar;57(3):403–405. doi: 10.1104/pp.57.3.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jackson M. B., Osborne D. J. Ethylene, the natural regulator of leaf abscission. Nature. 1970 Mar 14;225(5237):1019–1022. doi: 10.1038/2251019a0. [DOI] [PubMed] [Google Scholar]
  8. Jiao X. Z., Philosoph-Hadas S., Su L. Y., Yang S. F. The Conversion of 1-(Malonylamino)cyclopropane-1-Carboxylic Acid to 1-Aminocyclopropane-1-Carboxylic Acid in Plant Tissues. Plant Physiol. 1986 Jun;81(2):637–641. doi: 10.1104/pp.81.2.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jordan W. R., Morgan P. W., Davenport T. L. Water Stress Enhances Ethylene-mediated Leaf Abscission in Cotton. Plant Physiol. 1972 Dec;50(6):756–758. doi: 10.1104/pp.50.6.756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kiss H. G., Koning R. E. Endogenous Levels and Transport of 1-Aminocyclopropane-1-Carboxylic Acid in Stamens of Ipomoea nil (Convolvulaceae). Plant Physiol. 1989 May;90(1):157–161. doi: 10.1104/pp.90.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lizada M. C., Yang S. F. A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. Anal Biochem. 1979 Nov 15;100(1):140–145. doi: 10.1016/0003-2697(79)90123-4. [DOI] [PubMed] [Google Scholar]
  12. Morgan P. W., He C. J., De Greef J. A., De Proft M. P. Does water deficit stress promote ethylene synthesis by intact plants? Plant Physiol. 1990 Dec;94(4):1616–1624. doi: 10.1104/pp.94.4.1616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Narayana I., Lalonde S., Saini H. S. Water-stress-induced ethylene production in wheat : a fact or artifact? Plant Physiol. 1991 Jun;96(2):406–410. doi: 10.1104/pp.96.2.406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scholander P. F., Bradstreet E. D., Hemmingsen E. A., Hammel H. T. Sap Pressure in Vascular Plants: Negative hydrostatic pressure can be measured in plants. Science. 1965 Apr 16;148(3668):339–346. doi: 10.1126/science.148.3668.339. [DOI] [PubMed] [Google Scholar]
  15. Woltering E. J. Interorgan translocation of 1-aminocyclopropane-1-carboxylic Acid and ethylene coordinates senescence in emasculated cymbidium flowers. Plant Physiol. 1990 Mar;92(3):837–845. doi: 10.1104/pp.92.3.837. [DOI] [PMC free article] [PubMed] [Google Scholar]

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