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. 1992 Sep;100(1):397–402. doi: 10.1104/pp.100.1.397

Purification of an Infection-Related, Extracellular Peroxidase from Barley 1

Kent Kerby 1,2,2, Shauna C Somerville 1,2
PMCID: PMC1075564  PMID: 16652974

Abstract

Increases in two extracellular peroxidases were observed following inoculation of barley (Hordeum vulgare L.) with the powdery mildew pathogen (Erysiphe graminis DC.: Fr. f. sp. hordei Em. Marchal). The more prominent isozyme, P8.5, was purified from intercellular wash fluids by acetone precipitation, ion-exchange chromatography, isoelectric focusing, and gel filtration. Purified P8.5 is a heme-containing, glycoprotein with a Mr of 35,000. It has eight cysteine residues. A highly specific, high-titer antiserum to deglycosylated P8.5 was produced.

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Selected References

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