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. 1992 Sep;100(1):529–532. doi: 10.1104/pp.100.1.529

A New Method of Measuring Protein-Methionine-S-Oxide Reductase Activity 1

David L Ferguson 1,2, John J Burke 1,2
PMCID: PMC1075582  PMID: 16652994

Abstract

A new assay for measuring protein-methionine-S-oxide reductase is reported. The assay measures the conversion of N-(9-fluorenylmethoxycarbonyl)-methionine sulfoxide to N-(9-fluorenylmethoxycarbonyl)-methionine using fluorescence detection after high-performance liquid chromatography separation. Enzyme activity is linear over a 60-min period and the assay is sensitive enough to detect the consumption of only 1% of available substrate. Protein-methionine-S-oxide reductase activity was demonstrated in pea (Pisum sativum L.) chloroplasts, and enzyme levels in leaves of etiolated and light-grown seedlings were compared.

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Selected References

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