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. 1992 Oct;100(2):644–650. doi: 10.1104/pp.100.2.644

Effects of Nitrite, Chlorate, and Chlorite on Nitrate Uptake and Nitrate Reductase Activity 1

M Yaeesh Siddiqi 1, Bryan J King 1, Anthony D M Glass 1
PMCID: PMC1075607  PMID: 16653041

Abstract

Effects of NO2−, ClO3−, and ClO2− on the induction of nitrate transport and nitrate reductase activity (NRA) as well as their effects on NO3− influx into roots of intact barley (Hordeum vulgare cv Klondike) seedlings were investigated. A 24-h pretreatment with 0.1 mol m−3 NO2− fully induced NO3− transport but failed to induce NRA. Similar pretreatments with ClO3− and ClO2− induced neither NO3− transport nor NRA. Net ClO3− uptake was induced by NO3− but not by ClO3− itself, indicating that NO3− and ClO3− transport occur via the NO3− carrier. At the uptake step, NO2− and ClO2− strongly inhibited NO3− influx; the former exhibited classical competitive kinetics, whereas the latter exhibited complex mixed-type kinetics. ClO3− proved to be a weak inhibitor of NO3− influx (Ki = 16 mol m−3) in a noncompetitive manner. The implications of these findings are discussed in the context of the suitability of these NO3− analogs as screening agents for the isolation of mutants defective in NO3− transport.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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