Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1992 Oct;100(2):908–914. doi: 10.1104/pp.100.2.908

Light Regulation of Leaf Mitochondrial Pyruvate Dehydrogenase Complex 1

Role of Photorespiratory Carbon Metabolism

Joanna Gemel 1, Douglas D Randall 1
PMCID: PMC1075643  PMID: 16653075

Abstract

Light-dependent inactivation of mitochondrial pyruvate dehydrogenase complex (mtPDC) in pea (Pisum sativum L.) leaves was further characterized, and this phenomenon was extended to several monocot and dicot species. The light-dependent inactivation of mtPDC in vivo was rapidly reversed in the dark, even after prolonged illumination. The mtPDC can be efficiently cycled through the inactivated-reactivated status by rapid light-dark cycling. Light-dependent inactivation of mtPDC was shown to be suppressed by inhibitors of photorespiratory carbon metabolism, including 2-pyridylhydroxymethane sulfonate, isonicotinic acid hydrazide, and aminoacetonitrile, and by an inhibitor of photosynthesis, 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Glycine fed to pea leaf strips in the dark yielded partially inactivated leaf mtPDC, and this inactivation was blocked by inhibitors of glycine oxidation. It is concluded that the photorespiratory glycine to serine conversion that occurs in C3 leaf mitochondria can provide the NADH to drive oxidative phosphorylation and subsequent inactivation of mtPDC. Glycine oxidation also produces ammonium ion, which has been shown to enhance the inactivation of mtPDC in vitro by stimulating the pyruvate dehydrogenase kinase that catalyzes the phosphorylation (inactivation) of the mtPDC. Thus, light-dependent, photorespiration-stimulated inactivation of the mtPDC can regulate carbon entry into the Krebs cycle during C3 photosynthesis.

Full text

PDF
908

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Budde R. J., Fang T. K., Randall D. D. Regulation of the phosphorylation of mitochondrial pyruvate dehydrogenase complex in situ: effects of respiratory substrates and calcium. Plant Physiol. 1988 Dec;88(4):1031–1036. doi: 10.1104/pp.88.4.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Budde R. J., Randall D. D. Light as a signal influencing the phosphorylation status of plant proteins. Plant Physiol. 1990 Dec;94(4):1501–1504. doi: 10.1104/pp.94.4.1501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Budde R. J., Randall D. D. Pea leaf mitochondrial pyruvate dehydrogenase complex is inactivated in vivo in a light-dependent manner. Proc Natl Acad Sci U S A. 1990 Jan;87(2):673–676. doi: 10.1073/pnas.87.2.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burnell J. N., Hatch M. D. Dark-light regulation of pyruvate, Pi dikinase in C4 plants: evidence that the same protein catalyses activation and inactivation. Biochem Biophys Res Commun. 1983 Feb 28;111(1):288–293. doi: 10.1016/s0006-291x(83)80149-1. [DOI] [PubMed] [Google Scholar]
  5. Camp P. J., Randall D. D. Purification and Characterization of the Pea Chloroplast Pyruvate Dehydrogenase Complex : A Source of Acetyl-CoA and NADH for Fatty Acid Biosynthesis. Plant Physiol. 1985 Mar;77(3):571–577. doi: 10.1104/pp.77.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Campbell W. J., Ogren W. L. A novel role for light in the activation of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol. 1990 Jan;92(1):110–115. doi: 10.1104/pp.92.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gardeström P., Wigge B. Influence of Photorespiration on ATP/ADP Ratios in the Chloroplasts, Mitochondria, and Cytosol, Studied by Rapid Fractionation of Barley (Hordeum vulgare) Protoplasts. Plant Physiol. 1988 Sep;88(1):69–76. doi: 10.1104/pp.88.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huber J. L., Huber S. C., Nielsen T. H. Protein phosphorylation as a mechanism for regulation of spinach leaf sucrose-phosphate synthase activity. Arch Biochem Biophys. 1989 May 1;270(2):681–690. doi: 10.1016/0003-9861(89)90551-1. [DOI] [PubMed] [Google Scholar]
  9. Jiao J. A., Chollet R. Posttranslational regulation of phosphoenolpyruvate carboxylase in c(4) and crassulacean Acid metabolism plants. Plant Physiol. 1991 Apr;95(4):981–985. doi: 10.1104/pp.95.4.981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kirschbaum M. U., Farquhar G. D. Investigation of the CO(2) Dependence of Quantum Yield and Respiration in Eucalyptus pauciflora. Plant Physiol. 1987 Apr;83(4):1032–1036. doi: 10.1104/pp.83.4.1032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Krömer S., Heldt H. W. On the Role of Mitochondrial Oxidative Phosphorylation in Photosynthesis Metabolism as Studied by the Effect of Oligomycin on Photosynthesis in Protoplasts and Leaves of Barley (Hordeum vulgare). Plant Physiol. 1991 Apr;95(4):1270–1276. doi: 10.1104/pp.95.4.1270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McCashin B. G., Cossins E. A., Canvin D. T. Dark Respiration during Photosynthesis in Wheat Leaf Slices. Plant Physiol. 1988 May;87(1):155–161. doi: 10.1104/pp.87.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miernyk J. A., Randall D. D. Some kinetic and regulatory properties of the pea mitochondrial pyruvate dehydrogenase complex. Plant Physiol. 1987 Feb;83(2):306–310. doi: 10.1104/pp.83.2.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miernyk J. A., Randall D. D. Some properties of pea mitochondrial phospho-pyruvate dehydrogenase-phosphatase. Plant Physiol. 1987 Feb;83(2):311–315. doi: 10.1104/pp.83.2.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Randall D. D., Miernyk J. A., Fang T. K., Budde R. J., Schuller K. A. Regulation of the pyruvate dehydrogenase complexes in plants. Ann N Y Acad Sci. 1989;573:192–205. doi: 10.1111/j.1749-6632.1989.tb14997.x. [DOI] [PubMed] [Google Scholar]
  16. Randall D. D., Williams M., Rapp B. J. Phosphorylation-dephosphorylation of pyruvate dehydrogenase complex from pea leaf mitochondria. Arch Biochem Biophys. 1981 Apr 1;207(2):437–444. doi: 10.1016/0003-9861(81)90051-5. [DOI] [PubMed] [Google Scholar]
  17. Schuller K. A., Randall D. D. Mechanism of pyruvate inhibition of plant pyruvate dehydrogenase kinase and synergism with ADP. Arch Biochem Biophys. 1990 Apr;278(1):211–216. doi: 10.1016/0003-9861(90)90250-3. [DOI] [PubMed] [Google Scholar]
  18. Schuller K. A., Randall D. D. Regulation of pea mitochondrial pyruvate dehydrogenase complex : does photorespiratory ammonium influence mitochondrial carbon metabolism? Plant Physiol. 1989 Apr;89(4):1207–1212. doi: 10.1104/pp.89.4.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Servaites J. C. Chemical inhibition of the glycolate pathway in soybean leaf cells. Plant Physiol. 1977 Oct;60(4):461–466. doi: 10.1104/pp.60.4.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wiskich J. T., Bryce J. H., Day D. A., Dry I. B. Evidence for Metabolic Domains within the Matrix Compartment of Pea Leaf Mitochondria : Implications for Photorespiratory Metabolism. Plant Physiol. 1990 Jun;93(2):611–616. doi: 10.1104/pp.93.2.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. ZELITCH I. The relationship of glycolic acid to respiration and photosynthesis in tobacco leaves. J Biol Chem. 1959 Dec;234:3077–3081. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES