Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1992 Nov;100(3):1291–1295. doi: 10.1104/pp.100.3.1291

Monovinyl and Divinyl Protochlorophyllide Pools in Etiolated Tissues of Higher Plants 1

Yuzo Shioi 1,2, Ken-ichiro Takamiya 1,2
PMCID: PMC1075780  PMID: 16653119

Abstract

The composition of chlorophyll-precursor pigments, particularly the contents of monovinyl (MV) and divinyl (DV) protochlorophyllides (Pchlides), in etiolated tissues of higher plants were determined by polyethylene-column HPLC (Y. Shioi, S. I. Beale [1987] Anal Biochem 162: 493-499), which enables the complete separation of these pigments. DV-Pchlide was ubiquitous in etiolated tissue of higher plants. From the analyses of 24 plant species belonging to 17 different families, it was shown that the concentration of DV-Pchlide was strongly dependent on the plant species and the age of the plants. The ratio of DV-Pchlide to MV-Pchlide in high DV-Pchlide plants such as cucumber and leaf mustard decreased sharply with increasing age. Levels of DV-Pchlide in Gramineae plants were considerably lower at all ages compared with those of other plants. Etiolated tissues of higher plants such as barley and corn were, therefore, good sources of MV-Pchlide. Absorption spectra of the purified MV- and DV-Pchlides in ether are presented and compared.

Full text

PDF
1291

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belanger F. C., Rebeiz C. A. Chloroplast biogenesis. Detection of divinyl protochlorophyllide in higher plants. J Biol Chem. 1980 Feb 25;255(4):1266–1272. [PubMed] [Google Scholar]
  2. JONES O. T. MAGNESIUM 2,4-DIVINYLPHAEOPORPHYRIN A5 MONOMETHYL ESTER, A PROTOCHLOROPHYLL-LIKE PIGMENT PRODUCED BY RHODOPSEUDOMONAS SPHEROIDES. Biochem J. 1963 Nov;89:182–189. doi: 10.1042/bj0890182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Lancer H. A., Cohen C. E., Schiff J. A. Changing ratios of phototransformable protochlorophyll and protochlorophyllide of bean seedlings developing in the dark. Plant Physiol. 1976 Mar;57(3):369–374. doi: 10.1104/pp.57.3.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Shioi Y., Beale S. I. Polyethylene-based high-performance liquid chromatography of chloroplast pigments: resolution of mono- and divinyl chlorophyllides and other pigment mixtures. Anal Biochem. 1987 May 1;162(2):493–499. doi: 10.1016/0003-2697(87)90425-8. [DOI] [PubMed] [Google Scholar]
  5. Shioi Y., Doi M., Böddi B. Selective inhibition of chlorophyll biosynthesis by nicotinamide. Arch Biochem Biophys. 1988 Nov 15;267(1):69–74. doi: 10.1016/0003-9861(88)90009-4. [DOI] [PubMed] [Google Scholar]
  6. Shioi Y., Sasa T. Compositional heterogeneity of protochlorophyllide ester in etiolated leaves of higher plants. Arch Biochem Biophys. 1983 Jan;220(1):286–292. doi: 10.1016/0003-9861(83)90412-5. [DOI] [PubMed] [Google Scholar]
  7. Tripathy B. C., Rebeiz C. A. Chloroplast Biogenesis 60 : Conversion of Divinyl Protochlorophyllide to Monovinyl Protochlorophyllide in Green(ing) Barley, a Dark Monovinyl/Light Divinyl Plant Species. Plant Physiol. 1988 May;87(1):89–94. doi: 10.1104/pp.87.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tripathy B. C., Rebeiz C. A. Chloroplast biogenesis. Demonstration of the monovinyl and divinyl monocarboxylic routes of chlorophyll biosynthesis in higher plants. J Biol Chem. 1986 Oct 15;261(29):13556–13564. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES