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. 1992 Nov;100(3):1471–1478. doi: 10.1104/pp.100.3.1471

Induction of a Putative Ca2+-ATPase mRNA in NaCl-Adapted Cells 1

Eva Perez-Prat 1,2,3, Meena L Narasimhan 1,2,3, Marla L Binzel 1,2,3, Miguel A Botella 1,2,3, Zutang Chen 1,2,3, Victoriano Valpuesta 1,2,3, Ray A Bressan 1,2,3, Paul M Hasegawa 1,2,3
PMCID: PMC1075808  PMID: 16653146

Abstract

A cDNA clone was isolated that encodes the partial sequence of a putative endoplasmic reticulum Ca2+-ATPase of tobacco. The 1.497-kb insert had an open reading frame of 1.149 kb. The deduced peptide had the greatest homology to the endoplasmic reticulum Ca2+-ATPases of Drosophila and Artemia, followed by the mammalian and avian enzymes (SERCA2 and 3). The cDNA insert hybridized to a single mRNA of 4.4 kb from tobacco cultured cells or plant tissues. The level of this transcript was induced about 2-fold by NaCl shock in 428 mm NaCl-deadapted tobacco cells that were maintained in medium without salt, but not in unadapted cells. The level of this transcript was 3- to 4-fold higher in 428 mm NaCl-adapted cells growing in salt than in unadapted cells growing without salt.

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