Abstract
When supplied under low chloride concentrations, vanadate inhibits the blue light-stimulated swelling of Vicia faba L. guard cell protoplasts in a dose-dependent fashion. The volume of guard cell protoplasts incubated in 10 mm K-imino-diacetic acid, 0.4 m mannitol, and 1 mm CaCl2 remained essentially constant under 1000 μmol m−2 s−1 red light, but increased an average of 27% after 8 min of the addition of 50 μmol m−2 s−1 blue light to the background red light. At 500 μm, vanadate completely inhibits the response to blue light. Vanadate also inhibits the swelling of guard cell protoplasts stimulated by the H+-ATPase agonist fusicoccin. The vanadate sensitivity of the blue light-stimulated swelling implicates a proton-pumping ATPase as a component of the sensory transduction of blue light in guard cells.
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- Gallagher S. R., Leonard R. T. Effect of vanadate, molybdate, and azide on membrane-associated ATPase and soluble phosphatase activities of corn roots. Plant Physiol. 1982 Nov;70(5):1335–1340. doi: 10.1104/pp.70.5.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gautier H., Vavasseur A., Lascève G., Boudet A. M. Redox Processes in the Blue Light Response of Guard Cell Protoplasts of Commelina communis L. Plant Physiol. 1992 Jan;98(1):34–38. doi: 10.1104/pp.98.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raschke K., Schnabl H. Availability of Chloride Affects the Balance between Potassium Chloride and Potassium Malate in Guard Cells of Vicia faba L. Plant Physiol. 1978 Jul;62(1):84–87. doi: 10.1104/pp.62.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubinson K. A. Concerning the form of biochemically active vanadium. Proc R Soc Lond B Biol Sci. 1981 May 7;212(1186):65–84. doi: 10.1098/rspb.1981.0025. [DOI] [PubMed] [Google Scholar]
- Serrano E. E., Zeiger E., Hagiwara S. Red light stimulates an electrogenic proton pump in Vicia guard cell protoplasts. Proc Natl Acad Sci U S A. 1988 Jan;85(2):436–440. doi: 10.1073/pnas.85.2.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Serrano E. E., Zeiger E. Sensory transduction and electrical signaling in guard cells. Plant Physiol. 1989 Nov;91(3):795–799. doi: 10.1104/pp.91.3.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Srivastava A., Zeiger E. Fast Fluorescence Quenching from Isolated Guard Cell Chloroplasts of Vicia faba Is Induced by Blue Light and Not by Red Light. Plant Physiol. 1992 Nov;100(3):1562–1566. doi: 10.1104/pp.100.3.1562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vani T., Raghavendra A. S. Tetrazolium Reduction by Guard Cells in Abaxial Epidermis of Vicia faba: Blue Light Stimulation of a Plasmalemma Redox System. Plant Physiol. 1989 May;90(1):59–62. doi: 10.1104/pp.90.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeiger E., Hepler P. K. Light and stomatal function: blue light stimulates swelling of guard cell protoplasts. Science. 1977 May 20;196(4292):887–889. doi: 10.1126/science.196.4292.887. [DOI] [PubMed] [Google Scholar]