Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1992 Nov;100(3):1580–1583. doi: 10.1104/pp.100.3.1580

Purification and Characterization of Cytosolic 6-Phosphogluconate Dehydrogenase Isozymes from Maize 1

Julia Bailey-Serres 1, Minh T Nguyen 1
PMCID: PMC1075824  PMID: 16653162

Abstract

Cytosolic isozymes of 6-phosphogluconate dehydrogenase were purified from roots of maize (Zea mays L.). The final preparation contained two 55-kD proteins. Affinity-purified dehydrogenases from a maize line that is null for both cytosolic 6-phosphogluconate dehydrogenase isozymes (Pgd1-null, Pgd2-null) lacked the 55-kD proteins. The substrate kinetics of the purified enzyme were determined.

Full text

PDF
1580

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey-Serres J., Tom J., Freeling M. Expression and distribution of cytosolic 6-phosphogluconate dehydrogenase isozymes in maize. Biochem Genet. 1992 Jun;30(5-6):233–246. doi: 10.1007/BF02396214. [DOI] [PubMed] [Google Scholar]
  2. Gottlieb L. D. Conservation and duplication of isozymes in plants. Science. 1982 Apr 23;216(4544):373–380. doi: 10.1126/science.216.4544.373. [DOI] [PubMed] [Google Scholar]
  3. Kato N., Sahm H., Schütte H., Wagner F. Purification and properties of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from a methanol-utilizing yeast, Candida boidinii. Biochim Biophys Acta. 1979 Jan 12;566(1):1–11. doi: 10.1016/0005-2744(79)90242-0. [DOI] [PubMed] [Google Scholar]
  4. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  5. Rosemeyer M. A. The biochemistry of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and glutathione reductase. Cell Biochem Funct. 1987 Apr;5(2):79–95. doi: 10.1002/cbf.290050202. [DOI] [PubMed] [Google Scholar]
  6. Schnarrenberger C., Oeser A., Tolbert N. E. Two isoenzymes each of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in spinach leaves. Arch Biochem Biophys. 1973 Jan;154(1):438–448. doi: 10.1016/0003-9861(73)90077-5. [DOI] [PubMed] [Google Scholar]
  7. Schnarrenberger C., Tetour M., Herbert M. Development and intracellular distribution of enzymes of the oxidative pentose phosphate cycle in radish cotyledons. Plant Physiol. 1975 Dec;56(6):836–840. doi: 10.1104/pp.56.6.836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Simcox P. D., Dennis D. T. 6-Phosphogluconate Dehydrogenase Isoenzymes from the Developing Endosperm of Ricinus communis L. Plant Physiol. 1978 Aug;62(2):287–290. doi: 10.1104/pp.62.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES