Abstract
Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cullimore J. V., Gebhardt C., Saarelainen R., Miflin B. J., Idler K. B., Barker R. F. Glutamine synthetase of Phaseolus vulgaris L.: organ-specific expression of a multigene family. J Mol Appl Genet. 1984;2(6):589–599. [PubMed] [Google Scholar]
- Edwards J. W., Walker E. L., Coruzzi G. M. Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase. Proc Natl Acad Sci U S A. 1990 May;87(9):3459–3463. doi: 10.1073/pnas.87.9.3459. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirel B., Weatherley C., Cretin C., Bergounioux C., Gadal P. Multiple Subunit Composition of Chloroplastic Glutamine Synthetase of Nicotiana tabacum L. Plant Physiol. 1984 Feb;74(2):448–450. doi: 10.1104/pp.74.2.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- McNally S. F., Hirel B., Gadal P., Mann A. F., Stewart G. R. Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf. Plant Physiol. 1983 May;72(1):22–25. doi: 10.1104/pp.72.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tingey S. V., Coruzzi G. M. Glutamine Synthetase of Nicotiana plumbaginifolia: Cloning and in Vivo Expression. Plant Physiol. 1987 Jun;84(2):366–373. doi: 10.1104/pp.84.2.366. [DOI] [PMC free article] [PubMed] [Google Scholar]