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. 1992 Dec;100(4):1780–1786. doi: 10.1104/pp.100.4.1780

The Effect of Heat Shock on Morphogenesis in Barley 1

Coordinated Circadian Regulation of mRNA Levels for Light-Regulated Genes and of the Capacity for Accumulation of Chlorophyll Protein Complexes

Jens Beator 1, Eyck Pötter 1, Klaus Kloppstech 1
PMCID: PMC1075864  PMID: 16653197

Abstract

The effect of daily heat-shock treatments on gene expression and morphogenesis of etiolated barley (Hordeum vulgare) was investigated. Heat-shock treatments in the dark induced shortening of the primary leaves and the coleoptiles to the length of those in light-grown plantlets. In addition, the mRNA levels of the light-induced genes that were investigated were raised under these conditions and showed distinct oscillations over a period of at least 3 d. While the mRNA levels for chlorophyll a/b binding protein (LHC II), plastocyanin, and the small subunit of ribulose-1,5-bisphosphate carboxylase had maxima between 8 and 12 pm (12-16h after the last heat-shock treatment), the mRNA levels for thionin oscillated with a phase opposed to that of LHC II. Etiolated barley, the circadian oscillator of which was synchronized by cyclic heatshock treatments, was illuminated for a constant interval at different times of the day; this led to the finding that greening was fastest at the time when the maximal levels of mRNA for LHC II were also observed. Whereas accumulation of chlorophyll a during a 4-h period of illumination oscillated by a factor of 3, chlorophyll b accumulation changed 10- to 15-fold. Similarly, accumulation of LHC II was highest when pigments accumulated maximally. Hence, greening or, in other words, thylakoid membrane assembly is under control of the circadian oscillator.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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