Abstract
In leaves of tomato (Lycopersicon esculentum), the synthesis of a light-harvesting complex (LHC) polypeptide of photosystem II and the quinone B (QB)-binding protein varies at different time points during the day. In vivo labeling with [35S]methionine revealed diurnal oscillations of synthesis of these thylakoid membrane proteins. Both proteins are synthesized at elevated levels right after the transition from darkness to light, a maximum is reached around noon, and decreasing levels were measured during the afternoon and night. In addition, in constant darkness both proteins were also synthesized to varied extents at different diurnal time points. Together, these results indicate that the synthesis of a LHC II and the QB-binding protein is under the control of the circadian clock. This circadian oscillation of LHC II protein synthesis correlates with the very well documented circadian Lhc a/b mRNA accumulation.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adamska I., Scheel B., Kloppstech K. Circadian oscillations of nuclear-encoded chloroplast proteins in pea (Pisum sativum). Plant Mol Biol. 1991 Nov;17(5):1055–1065. doi: 10.1007/BF00037144. [DOI] [PubMed] [Google Scholar]
- Anderson J. M., Andersson B. The dynamic photosynthetic membrane and regulation of solar energy conversion. Trends Biochem Sci. 1988 Sep;13(9):351–355. doi: 10.1016/0968-0004(88)90106-5. [DOI] [PubMed] [Google Scholar]
- Barber J., Andersson B. Too much of a good thing: light can be bad for photosynthesis. Trends Biochem Sci. 1992 Feb;17(2):61–66. doi: 10.1016/0968-0004(92)90503-2. [DOI] [PubMed] [Google Scholar]
- Bennett J. Biosynthesis of the light-harvesting chlorophyll a/b protein. Polypeptide turnover in darkness. Eur J Biochem. 1981 Aug;118(1):61–70. doi: 10.1111/j.1432-1033.1981.tb05486.x. [DOI] [PubMed] [Google Scholar]
- Busheva M., Garab G., Liker E., Tóth Z., Szèll M., Nagy F. Diurnal Fluctuations in the Content and Functional Properties of the Light Harvesting Chlorophyll a/b Complex in Thylakoid Membranes : Correlation with the Diurnal Rhythm of the mRNA Level. Plant Physiol. 1991 Apr;95(4):997–1003. doi: 10.1104/pp.95.4.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang Y. C., Walling L. L. Abscisic Acid Negatively Regulates Expression of Chlorophyll a/b Binding Protein Genes during Soybean Embryogeny. Plant Physiol. 1991 Nov;97(3):1260–1264. doi: 10.1104/pp.97.3.1260. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green B. R., Pichersky E., Kloppstech K. Chlorophyll a/b-binding proteins: an extended family. Trends Biochem Sci. 1991 May;16(5):181–186. doi: 10.1016/0968-0004(91)90072-4. [DOI] [PubMed] [Google Scholar]
- Ohad I., Kyle D. J., Hirschberg J. Light-dependent degradation of the Q(B)-protein in isolated pea thylakoids. EMBO J. 1985 Jul;4(7):1655–1659. doi: 10.1002/j.1460-2075.1985.tb03833.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Piechulla B., Gruissem W. Diurnal mRNA fluctuations of nuclear and plastid genes in developing tomato fruits. EMBO J. 1987 Dec 1;6(12):3593–3599. doi: 10.1002/j.1460-2075.1987.tb02690.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riesselmann S., Piechulla B. Effect of dark phases and temperature on the chlorophyll a/b binding protein mRNA level oscillations in tomato seedlings. Plant Mol Biol. 1990 Apr;14(4):605–616. doi: 10.1007/BF00027506. [DOI] [PubMed] [Google Scholar]