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. 1992 Dec;100(4):2008–2012. doi: 10.1104/pp.100.4.2008

Electrically Induced Protoplast Fusion Using Pulse Electric Fields for Dielectrophoresis 1

Antoaneta P Dimitrova 1, Alexander M Christov 1,2
PMCID: PMC1075898  PMID: 16653231

Abstract

The formation of protoplast chains in suspensions of isolated pea (Pisum sativum cv Ran 1) mesophyll protoplasts induced by electric fields was studied. The chain formation induced by a sine-wave field (2 V, peak to peak; 500-0.1 kHz) was compared to that induced by an alternating pulse field (1 V, amplitude; 0.1-0.4 kHz). An increased number of dielectrophoretically paired protoplasts, formation of protoplast chains in the presence of CaCl2 up to 5 mm, and protoplast fusion in the presence of 3 mm CaCl2 were found when the pulse field was applied. The present results suggest the possibility of electrically induced protoplast fusion at cation concentrations that prevent fusion when sine-wave fields are applied.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bates G. W., Gaynor J. J., Shekhawat N. S. Fusion of plant protoplasts by electric fields. Plant Physiol. 1983 Aug;72(4):1110–1113. doi: 10.1104/pp.72.4.1110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Christov A. M., Vaklinova S. G. Effects of prostaglandins e(2) and f(2alpha) on the electrofusion of pea mesophyll protoplasts. Plant Physiol. 1987 Mar;83(3):500–504. doi: 10.1104/pp.83.3.500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Klöck G., Zimmermann U. Facilitated electrofusion of vacuolated x evacuolated oat mesophyll protoplasts in hypo-osmolar media after alignment with an alternating field of modulated strength. Biochim Biophys Acta. 1990 Jun 11;1025(1):87–93. doi: 10.1016/0005-2736(90)90194-s. [DOI] [PubMed] [Google Scholar]
  4. Pohl H. A., Crane J. S. Dielectrophoresis of cells. Biophys J. 1971 Sep;11(9):711–727. doi: 10.1016/S0006-3495(71)86249-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Saunders J. A., Roskos L. A., Mischke S., Aly M. A., Owens L. D. Behavior and viability of tobacco protoplasts in response to electrofusion parameters. Plant Physiol. 1986 Jan;80(1):117–121. doi: 10.1104/pp.80.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Teissie J., Rols M. P. Fusion of mammalian cells in culture is obtained by creating the contact between cells after their electropermeabilization. Biochem Biophys Res Commun. 1986 Oct 15;140(1):258–266. doi: 10.1016/0006-291x(86)91084-3. [DOI] [PubMed] [Google Scholar]
  7. Watts J. W., King J. M. A simple method for large-scale electrofusion and culture of plant protoplasts. Biosci Rep. 1984 Apr;4(4):335–342. doi: 10.1007/BF01140497. [DOI] [PubMed] [Google Scholar]
  8. Zimmermann U. Electric field-mediated fusion and related electrical phenomena. Biochim Biophys Acta. 1982 Nov 30;694(3):227–277. doi: 10.1016/0304-4157(82)90007-7. [DOI] [PubMed] [Google Scholar]
  9. Zimmermann U., Vienken J. Electric field-induced cell-to-cell fusion. J Membr Biol. 1982;67(3):165–182. doi: 10.1007/BF01868659. [DOI] [PubMed] [Google Scholar]
  10. Zimmermann U., Vienken J., Pilwat G., Arnold W. M. Electro-fusion of cells: principles and potential for the future. Ciba Found Symp. 1984;103:60–85. doi: 10.1002/9780470720844.ch5. [DOI] [PubMed] [Google Scholar]

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