Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1992 Dec;100(4):2013–2017. doi: 10.1104/pp.100.4.2013

Organization of Ripening and Ethylene Regulatory Regions in a Fruit-Specific Promoter from Tomato (Lycopersicon esculentum) 1

Jill Deikman 1,2, Randy Kline 1, Robert L Fischer 1
PMCID: PMC1075899  PMID: 16653232

Abstract

Tomato (Lycopersicon esculentum) fruit ripening is initiated by an increase in ethylene hormone concentration. E8 gene transcription is fruit-specific and is activated at the onset of ripening and in unripe fruit treated with exogenous ethylene. To understand how E8 gene transcription is controlled during ripening, we analyzed the effect of deletions of flanking DNA sequences on E8 gene expression in transgenic tomato fruit. We found that a minimum of three 5′ and one 3′ regions influence E8 gene expression during fruit ripening. DNA sequences that confer responsiveness to exogenous ethylene in unripe fruit are distinct from DNA sequences that are sufficient for expression during fruit ripening.

Full text

PDF
2013

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Broglie K. E., Biddle P., Cressman R., Broglie R. Functional analysis of DNA sequences responsible for ethylene regulation of a bean chitinase gene in transgenic tobacco. Plant Cell. 1989 Jun;1(6):599–607. doi: 10.1105/tpc.1.6.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cass L. G., Kirven K. A., Christoffersen R. E. Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening. Mol Gen Genet. 1990 Aug;223(1):76–86. doi: 10.1007/BF00315799. [DOI] [PubMed] [Google Scholar]
  3. Cordes S., Deikman J., Margossian L. J., Fischer R. L. Interaction of a developmentally regulated DNA-binding factor with sites flanking two different fruit-ripening genes from tomato. Plant Cell. 1989 Oct;1(10):1025–1034. doi: 10.1105/tpc.1.10.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deikman J., Fischer R. L. Interaction of a DNA binding factor with the 5'-flanking region of an ethylene-responsive fruit ripening gene from tomato. EMBO J. 1988 Nov;7(11):3315–3320. doi: 10.1002/j.1460-2075.1988.tb03202.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dellapenna D., Alexander D. C., Bennett A. B. Molecular cloning of tomato fruit polygalacturonase: Analysis of polygalacturonase mRNA levels during ripening. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6420–6424. doi: 10.1073/pnas.83.17.6420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  7. Jones J. D., Dunsmuir P., Bedbrook J. High level expression of introduced chimaeric genes in regenerated transformed plants. EMBO J. 1985 Oct;4(10):2411–2418. doi: 10.1002/j.1460-2075.1985.tb03949.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klee H. J., Hayford M. B., Kretzmer K. A., Barry G. F., Kishore G. M. Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants. Plant Cell. 1991 Nov;3(11):1187–1193. doi: 10.1105/tpc.3.11.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lincoln J. E., Cordes S., Read E., Fischer R. L. Regulation of gene expression by ethylene during Lycopersicon esculentum (tomato) fruit development. Proc Natl Acad Sci U S A. 1987 May;84(9):2793–2797. doi: 10.1073/pnas.84.9.2793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lincoln J. E., Fischer R. L. Diverse mechanisms for the regulation of ethylene-inducible gene expression. Mol Gen Genet. 1988 Apr;212(1):71–75. doi: 10.1007/BF00322446. [DOI] [PubMed] [Google Scholar]
  11. Lincoln J. E., Fischer R. L. Regulation of Gene Expression by Ethylene in Wild-Type and rin Tomato (Lycopersicon esculentum) Fruit. Plant Physiol. 1988 Oct;88(2):370–374. doi: 10.1104/pp.88.2.370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Matzke M. A., Matzke A. J. Differential inactivation and methylation of a transgene in plants by two suppressor loci containing homologous sequences. Plant Mol Biol. 1991 May;16(5):821–830. doi: 10.1007/BF00015074. [DOI] [PubMed] [Google Scholar]
  13. McGarvey D. J., Sirevåg R., Christoffersen R. E. Ripening-related gene from avocado fruit : ethylene-inducible expression of the mRNA and polypeptide. Plant Physiol. 1992 Feb;98(2):554–559. doi: 10.1104/pp.98.2.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mol J. N., van der Krol A. R., van Tunen A. J., van Blokland R., de Lange P., Stuitje A. R. Regulation of plant gene expression by antisense RNA. FEBS Lett. 1990 Aug 1;268(2):427–430. doi: 10.1016/0014-5793(90)81298-3. [DOI] [PubMed] [Google Scholar]
  15. Napoli C., Lemieux C., Jorgensen R. Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. Plant Cell. 1990 Apr;2(4):279–289. doi: 10.1105/tpc.2.4.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oeller P. W., Lu M. W., Taylor L. P., Pike D. A., Theologis A. Reversible inhibition of tomato fruit senescence by antisense RNA. Science. 1991 Oct 18;254(5030):437–439. doi: 10.1126/science.1925603. [DOI] [PubMed] [Google Scholar]
  17. Penarrubia L., Aguilar M., Margossian L., Fischer R. L. An Antisense Gene Stimulates Ethylene Hormone Production during Tomato Fruit Ripening. Plant Cell. 1992 Jun;4(6):681–687. doi: 10.1105/tpc.4.6.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Raghothama K. G., Lawton K. A., Goldsbrough P. B., Woodson W. R. Characterization of an ethylene-regulated flower senescence-related gene from carnation. Plant Mol Biol. 1991 Jul;17(1):61–71. doi: 10.1007/BF00036806. [DOI] [PubMed] [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES