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[Preprint]. 2023 Dec 15:2023.12.15.571740. [Version 1] doi: 10.1101/2023.12.15.571740

Figure 5. BCG-disA-OE elicits greater interferon-β (IFN-β) responses than BCG-WT in primary murine and human macrophages in vitro.

Figure 5.

a. IRF3 induction measured in RAW-Lucia ISG reporter murine (Balb/c) macrophages. b. IFN-β levels in murine BMDM from wild-type and STING−/− mice (C57BL/6 background). C. IFN-β levels in J774.1 macrophages and human monocyte-derived macrophages (HMDM) following exposure to BCG strains. Cytokine levels were measured by ELISA after 24 hours exposure at and MOI of 20:1. Data are presented as mean values ± SD (n=3 biological replicates). Statistical analyses done using one-way ANOVA w/Tukey’s multiple comparisons test in panel a ; two-way ANOVA with Tukey’s test for multiple comparisons in panels b and d (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).