Table 1:
mesoSPIM v.5 vs Benchtop comparison.
| Version 5 | Benchtop | |
|---|---|---|
| Optical resolution, axial | 5.0 μm | 3.3 μm |
| Optical resolution, lateral | 2.7 μmr1 | 2.0 μm (1.5 to 2.6 μm)r2 |
| Magnification range | 0.63x – 6.3x | 0.9x – 20x |
| Detection objectives | Variable (motorized zoom) | Fixed-magnification (manual exchange) |
| Camera sensor diagonal (equiv. to FOV at 1x magnification) | 19 mm | 25 mm |
| Pixel size | 6.5 μm | 4.25 μm |
| Pixels/image | 4 MP | 15 MP |
| Footprint | 1 m2 | 0.25 m2 |
| Mobile | no | yes |
| Approximate parts cost, USD | 162,000c1 | 95,000c2 |
r1, resolution estimated with Olympus MVMPLAPO 1x objective at 4x zoom21.
r2, resolution averaged across three Mitutoyo BD Plan objectives: 5x: 2.6 μm, 10x, 1.8 μm, 20x, 1.5 μm, see SI Fig 1 for details.
c1, Version 5 costs include 4 laser lines (405, 488, 561, 638 nm) and optical table
c2, Benchtop cost estimate includes 3 laser lines (488, 561, 638 nm). Forth laser line (e.g. 405 nm) is optional, at additional cost of about 6,000 EUR. Magnifications included in the price: 2x, 5x, 10x.
Cost estimate does not include PC workstation and a screen.