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. Author manuscript; available in PMC: 2024 Jan 2.
Published in final edited form as: Biochemistry. 2023 Sep 20;62(24):3533–3547. doi: 10.1021/acs.biochem.3c00371

Figure 6. Transfection distance and efficiency after MC3 LNP-mediated Cas9 mRNA/Ai9 sgRNA delivery in striatum and hippocampus.

Figure 6.

(A, B) Transfection distance and total tdTomato+; DAPI+ cells in striatal injected samples (A), and hippocampal injected samples (B). (C, D) Quantification of tdTomato+; DAPI+ cells in every 12th brain slice in striatal injected samples (C), and hippocampal injected samples (D). Saline (n = 3 mice), low dose of Cas9 mRNA/Ai9 sgRNA (0.125 μg μL−1; Cas9 mRNA 1X; n = 2 mice), high dose of Cas9 mRNA/Ai9 sgRNA (0.250 μg μL−1; Cas9 mRNA 2X; n = 2 mice), low dose of MC3 LNP Cas9 mRNA/Ai9 sgRNA (0.125 μg μL−1; MC3 Cas9 mRNA 1X; n = 2 mice), and high dose of MC3 LNP Cas9 mRNA/Ai9 sgRNA (0.250 μg μL−1; MC3 Cas9 mRNA 2X; n = 3 mice).