Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 23;20(1):45–57. doi: 10.1080/15548627.2023.2247310

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

PMC Copyright notice

Figure 1. — HSC homeostasis is dependent on cell intrinsic autophagy. (A) Experimental setup for the inducible deletion of Atg5 in hematopoietic cells (Mx1^Cre atg5^fl/fl). (B) Gating strategy: representative dot plots of littermate controls (WT) and Mx1^Cre atg5^fl/fl SLAMF1/CD150⁺ CD48⁻ (HSCs) gated on Lin⁻ LY6A/Sca-1⁺ KIT/cKit⁺ (LSK) BM cells 2 weeks after poly(I:C) treatment. (C) Representative dot plots of WT and Vav^Cre atg7^fl/fl. (D) LSK frequency within the Lin⁻ population (left panel), HSC frequency within LSK population (middle panel) and HSC frequency within Lin⁻ population (right panel) in Mx1^Cre atg5^fl/fl (n = 8 mice/group). Data are represented as mean ± SEM with Mann-Whitney test. (E) LSK frequency within the Lin⁻ population (left panel), HSC frequency within LSK population (middle panel) and HSC frequency within Lin⁻ population (right panel) in Vav^Cre atg7^fl/fl (n = 45 mice/group). Data are represented as mean ± SEM with Mann-Whitney test. (F) Experimental setup of the tamoxifen-inducible deletion of Atg16l1 and Tomato reporter (Ai14) expression by Fdg5^Cre. (G) Atg16l1 transcript levels in Tomato⁺ LSKs. (H) Total number of Tomato⁺ HSCs (left and right hip and leg bones) three weeks post-tamoxifen treatment. Data are represented as mean ± SEM with two-tailed unpaired Student’s t test. Representative data from 1 out of 2 experiments (n_WT = 3, n_KO = 5). (I) Experimental setup for the generation of mixed bone marrow chimeras inducible for deletion of Atg5 (Mx1^Cre atg5^fl/fl). Lethally irradiated PTPRC^a/CD45.1 hosts reconstituted with a 1:1 mix of BM of Mx1^Cre atg5^fl/fl or WT BM (PTPRC^b/CD45.2) and PTPRC^a/CD45.1 WT BM. After 8 weeks, Atg5 deletion was induced by poly(I:C) and BM was analyzed at indicated times points after poly(I:C) administration. (J) Frequencies of PTPRC^a/CD45.1 cells and PTPRC^b/CD45.2 LSKs within Lin⁻ cells from mice reconstituted with PTPRC^a/CD45.1 WT:PTPRC^b/CD45.2 WT (white/gray) or with PTPRC^a/CD45.1 WT:PTPRC^b/CD45.2 Mx1^Cre atg5^fl/fl (white/black) BM. (K) Frequencies of HSCs within LSK in BM chimera. (J, K) data represented as mean ± SEM. n = 3–4 mice. Two-way ANOVA with post hoc Sidak’s test.