Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 23;20(1):45–57. doi: 10.1080/15548627.2023.2247310

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

PMC Copyright notice

Figure 4. — Rapamycin normalizes the metabolic status of autophagy deficient HSCs. (A) Experimental set-up of rapamycin (R) treatment in Vav^Cre atg7^fl/fl mice. (B) Representative images of long bones. (C) Weight and representative images of spleens. (D) LSK frequencies within live cells (left panel) and absolute numbers (right panel). (E) HSC frequencies within live (left panel) cells and absolute numbers (right panel). D-E: (n_WT = 17, n_WT+R = 13, n_KO = 13, n_KO+R = 14) (F) gMFI of p-RPS6 within HSCs (n_WT = 17, n_WT+R = 10, n_KO = 13, n_KO+R = 14). (G) gMFI of OPP within HSCs as a readout of translation (n_WT = 10, n_WT+R = 7, n_KO = 8, n_KO+R = 7). (H) gMFI of Kyn as a readout of AA uptake within HSCs (n_WT = 13, n_WT+R = 8, n_KO = 10, n_KO+R = 8). (I) gMFI of MKI67 within HSCs (n_WT = 13, n_WT+R = 10, n_KO = 10, n_KO+R = 7). D-E: pooled data from 3–5 independent experiments. Data are represented as mean ± SEM with two-way ANOVA with post hoc Tukey’s test.