Bacterial strains |
|
Pseudomonas aeruginosa |
Takeya K, Amako K. 1966 |
K |
Salmonella enterica |
Hoiseth and Stocker, 1981 |
SL1344 |
Listeria monocytogenes |
Edman et al. 1968 |
10403 |
Staphylococcus aureus |
Los Angeles County isolate, LAC |
USA300 |
|
Chemicals, peptides, and recombinant proteins |
|
Endothelial Growth Media 2 |
Lonza |
NC9525043 |
Trypsin EDTA |
ATCC |
50189662FP |
Trypsin Neutralizing Solution |
ATCC |
50189663FP |
Lennox L Broth |
Gibco |
10855021 |
Brain Heart Infusion Broth |
Sigma Aldrich |
53286 |
Lennox L Agar |
Invitrogen |
22700–025 |
Brain Heart Infusion Agar |
Research Products International |
B11500 |
Streptomycin |
Sigma Aldrich |
S1277 |
Two polydimethylsiloxane |
Electron Microscopy Sciences |
24236–10 |
Polyethyleneimine |
Sigma Aldrich |
03880 |
GA |
Sigma Aldrich |
G6257 |
Type 1 Rat Tail Collagen |
Corning |
354249 |
Paraformaldehyde |
Thermo Scientific |
AAJ19943K2 |
Phosphate Buffered Saline |
Thermo Scientific |
BP2944-100 |
Hoechst |
Sigma Aldrich |
23491-45-4 |
Phalloidin |
abcam |
ab176757 |
VE-Cadherin |
Miltenyi Biotech |
130-123-688 |
Dihydrorhodamine-123 |
Thermo Scientific |
D23806 |
IL-6 receptor blocking antibody |
R&D Systems |
MAB227; RRID: AB_2127908
|
IgG antibody |
R&D Systems |
HAF007; RRID: AB_357234
|
MIP-1 alpha receptor blocking antibody |
R&D Systems |
AF-270-SP; RRID: AB_354436
|
IgG antibody |
R&D Systems |
AB-108-C; RRID: AB_354267
|
|
Critical commercial assays |
|
Neutrophil Isolation Kit |
Miltenyi Biotec |
130-104-434 |
Erythrocyte Depletion Kit |
Miltenyi Biotec |
130-098-196 |
ProcartaPlex Human Inflammation Panel |
Invitrogen |
EPX200-12185-901 |
|
Experimental models: Cell lines |
|
Human Umbilical Vein Endothelial Cells |
Promocell |
50-305-964 |