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. 1990 Sep;94(1):35–39. doi: 10.1104/pp.94.1.35

Carbon Oxysulfide Is an Inhibitor of Both CO2 and HCO3− Uptake in the Cyanobacterium Synechococcus PCC7942

Murray R Badger 1, G Dean Price 1
PMCID: PMC1077185  PMID: 16667708

Abstract

Carbon oxysulfide (COS) was reinvestigated as an inhibitor of active inorganic carbon transport in cells of Synechococcus PCC7942 adapted to growth at low inorganic carbon. COS inhibited both CO2 and HCO3− transport processes in a reversible (in the short term) and mixed competitive manner. The inhibition of COS was established using both silicone oil centrifugation experiments and O2-evolution studies. The Ki for COS inhibition was 29 micromolar for CO2 transport and 110 micromolar for HCO3− transport. These results support a model of inorganic carbon transport with a central CO2 pump and an inducible HCO3− utilizing accessory protein which supplies CO2 to the primary pump.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badger M. R., Andrews T. J. Photosynthesis and Inorganic Carbon Usage by the Marine Cyanobacterium, Synechococcus sp. Plant Physiol. 1982 Aug;70(2):517–523. doi: 10.1104/pp.70.2.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Espie G. S., Miller A. G., Birch D. G., Canvin D. T. Simultaneous Transport of CO(2) and HCO(3) by the Cyanobacterium Synechococcus UTEX 625. Plant Physiol. 1988 Jul;87(3):551–554. doi: 10.1104/pp.87.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Furbank R. T., Badger M. R., Osmond C. B. Photosynthetic oxygen exchange in isolated cells and chloroplasts of c(3) plants. Plant Physiol. 1982 Oct;70(4):927–931. doi: 10.1104/pp.70.4.927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lorimer G. H., Pierce J. Carbonyl sulfide: an alternate substrate for but not an activator of ribulose-1,5-bisphosphate carboxylase. J Biol Chem. 1989 Feb 15;264(5):2764–2772. [PubMed] [Google Scholar]
  5. Miller A. G., Espie G. S., Canvin D. T. Active Transport of CO(2) by the Cyanobacterium Synechococcus UTEX 625 : Measurement by Mass Spectrometry. Plant Physiol. 1988 Mar;86(3):677–683. doi: 10.1104/pp.86.3.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Miller A. G., Espie G. S., Canvin D. T. Use of Carbon Oxysulfide, a Structural Analog of CO(2), to Study Active CO(2) Transport in the Cyanobacterium Synechococcus UTEX 625. Plant Physiol. 1989 Jul;90(3):1221–1231. doi: 10.1104/pp.90.3.1221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ogawa T., Togasaki R. K. Carbonyl sulfide: an inhibitor of inorganic carbon transport in cyanobacteria. Plant Physiol. 1988 Nov;88(3):800–804. doi: 10.1104/pp.88.3.800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Price G. D., Badger M. R. Ethoxyzolamide Inhibition of CO(2) Uptake in the Cyanobacterium Synechococcus PCC7942 without Apparent Inhibition of Internal Carbonic Anhydrase Activity. Plant Physiol. 1989 Jan;89(1):37–43. doi: 10.1104/pp.89.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Price G. D., Badger M. R. Ethoxyzolamide Inhibition of CO(2)-Dependent Photosynthesis in the Cyanobacterium Synechococcus PCC7942. Plant Physiol. 1989 Jan;89(1):44–50. doi: 10.1104/pp.89.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Price G. D., Badger M. R. Expression of Human Carbonic Anhydrase in the Cyanobacterium Synechococcus PCC7942 Creates a High CO(2)-Requiring Phenotype : Evidence for a Central Role for Carboxysomes in the CO(2) Concentrating Mechanism. Plant Physiol. 1989 Oct;91(2):505–513. doi: 10.1104/pp.91.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Volokita M., Zenvirth D., Kaplan A., Reinhold L. Nature of the Inorganic Carbon Species Actively Taken Up by the Cyanobacterium Anabaena variabilis. Plant Physiol. 1984 Nov;76(3):599–602. doi: 10.1104/pp.76.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]

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