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. 1990 Sep;94(1):380–383. doi: 10.1104/pp.94.1.380

Salt-Induced Dissociation of Carbonic Anhydrase from Intact Cells of Chlamydomonas reinhardtii1

H David Husic 1, Eileen A Quigley 1
PMCID: PMC1077236  PMID: 16667713

Abstract

The extracellular carbonic anhydrase of Chlamydomonas reinhardtii is dissociated from either intact or lysed cells by treatment with a 20 millimolar potassium phosphate buffer containing 0.4 molar KCI at pH 7.4. Electrophoretic analysis of proteins dissociated by the high salt treatment reveals that carbonic anhydrase comprises over 70% of the total released. These results suggest that the extracellular carbonic anhydrase in C. reinhardtii is bound to either the cell wall or plasma membrane through ionic interactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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