Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1990 Oct;94(2):479–484. doi: 10.1104/pp.94.2.479

Electron Transport through photosystem I Stimulates Light Activation of Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) by Rubisco Activase

William J Campbell 1,2, William L Ogren 1,2
PMCID: PMC1077257  PMID: 16667737

Abstract

The activation state of ribulose bisphosphate carboxylase/oxygenase (rubisco) in a lysed chloroplast system is increased by light in the presence of a saturating concentration of ATP and a physiological concentration of CO2 (10 micromolar). Electron transport inhibitors and artificial electron donors and acceptors were used to determine in which region of the photosynthetic electron transport chain this light-dependent reaction occurred. In the presence of DCMU and methyl viologen, the artificial donors durohydroquinone and 2,6-dichlorophenolindophenol (DCPIP) plus ascorbate both supported light activation of rubisco at saturating ATP concentrations. No light activation occurred when DCPIP was used as an acceptor with water as electron donor in the presence of ATP and dibromothymoquinone, even though photosynthetic electron transport was observed. Nigericin completely inhibited the light-dependent activation of rubisco. Based on these results, we conclude that stimulation of light activation of rubisco by rubisco activase requires electron transport through PSI but not PSII, and that this light requirement is not to supply the ATP needed by the rubisco activase reaction. Furthermore, a pH gradient across the thylakoid membrane appears necessary for maximum light activation of rubisco even when ATP is provided exogenously.

Full text

PDF
479

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bahr J. T., Jensen R. G. Activation of ribulose bisphosphate carboxylase in intact chloroplasts by CO2 and light. Arch Biochem Biophys. 1978 Jan 15;185(1):39–48. doi: 10.1016/0003-9861(78)90141-8. [DOI] [PubMed] [Google Scholar]
  3. Brooks A., Portis A. R., Sharkey T. D. Effects of Irradiance and Methyl Viologen Treatment on ATP, ADP, and Activation of Ribulose Bisphosphate Carboxylase in Spinach Leaves. Plant Physiol. 1988 Nov;88(3):850–853. doi: 10.1104/pp.88.3.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell W. J., Ogren W. L. A novel role for light in the activation of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol. 1990 Jan;92(1):110–115. doi: 10.1104/pp.92.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Izawa S., Pan R. L. Photosystem I electron transport and phosphorylation supported by electron donation to the plastoquinone region. Biochem Biophys Res Commun. 1978 Aug 14;83(3):1171–1177. doi: 10.1016/0006-291x(78)91518-8. [DOI] [PubMed] [Google Scholar]
  6. Perchorowicz J. T., Raynes D. A., Jensen R. G. Light limitation of photosynthesis and activation of ribulose bisphosphate carboxylase in wheat seedlings. Proc Natl Acad Sci U S A. 1981 May;78(5):2985–2989. doi: 10.1073/pnas.78.5.2985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Portis A. R., Jr Rubisco activase. Biochim Biophys Acta. 1990 Jan 4;1015(1):15–28. doi: 10.1016/0005-2728(90)90211-l. [DOI] [PubMed] [Google Scholar]
  8. Portis A. R., Salvucci M. E., Ogren W. L. Activation of Ribulosebisphosphate Carboxylase/Oxygenase at Physiological CO(2) and Ribulosebisphosphate Concentrations by Rubisco Activase. Plant Physiol. 1986 Dec;82(4):967–971. doi: 10.1104/pp.82.4.967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Robinson S. P., Portis A. R. Involvement of stromal ATP in the light activation of ribulose-1,5-bisphosphate carboxylase/oxygenase in intact isolated chloroplasts. Plant Physiol. 1988 Jan;86(1):293–298. doi: 10.1104/pp.86.1.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Salvucci M. E., Portis A. R., Ogren W. L. Light and CO(2) Response of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activation in Arabidopsis Leaves. Plant Physiol. 1986 Mar;80(3):655–659. doi: 10.1104/pp.80.3.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Streusand V. J., Portis A. R. Rubisco Activase Mediates ATP-Dependent Activation of Ribulose Bisphosphate Carboxylase. Plant Physiol. 1987 Sep;85(1):152–154. doi: 10.1104/pp.85.1.152. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES