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. 1990 Oct;94(2):704–709. doi: 10.1104/pp.94.2.704

Site of Clomazone Action in Tolerant-Soybean and Susceptible-Cotton Photomixotrophic Cell Suspension Cultures 1

Michael A Norman 1, Rex A Liebl 1, Jack M Widholm 1
PMCID: PMC1077288  PMID: 16667768

Abstract

Studies were conducted to determine the herbicidal site of clomazone action in tolerant-soybean (Glycine max [L.] Merr. cv Corsoy) (SB-M) and susceptible-cotton (Gossypium hirsutum [L.] cv Stoneville 825) (COT-M) photomixotrophic cell suspension cultures. Although a 10 micromolar clomazone treatment did not significantly reduce the terpene or mixed terpenoid content (microgram per gram fresh weight) of the SB-M cell line, there was over a 70% reduction in the chlorophyll (Chl), carotenoid (CAR), and plastoquinone (PQ) content of the COT-M cell line. The tocopherol (TOC) content was reduced only 35.6%. Reductions in the levels of Chl, CAR, TOC, and PQ indicate that the site of clomazone action in COT-M cells is prior to geranylgeranyl pyrophosphate (GGPP). The clomazone treatment did not significantly reduce the flow of [14C]mevalonate ([14C]MEV) (nanocuries per gram fresh weight) into CAR and the three mixed terpenoid compounds of SB-M cells. Conversely, [14C]MEV incorporation into CAR and the terpene moieties of Chl, PQ, and TOC in COT-M cells was reduced at least 73%, indicating that the site of clomazone action must be after MEV. Sequestration of clomazone away from the chloroplast cannot account for soybean tolerance to clomazone since chloroplasts isolated from both cell lines incubated with [14C]clomazone contained a similar amount of radioactivity (disintegrations per minute per microgram of Chl). The possible site(s) of clomazone inhibition include mevalonate kinase, phosphomevalonate kinase, pyrophosphomevalonate decarboxylase, isopentenyl pyrophosphate isomerase, and/or a prenyl transferase.

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Selected References

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  1. Desai I. D. Vitamin E analysis methods for animal tissues. Methods Enzymol. 1984;105:138–147. doi: 10.1016/s0076-6879(84)05019-9. [DOI] [PubMed] [Google Scholar]
  2. Heber U., Santarius K. A. Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z Naturforsch B. 1970 Jul;25(7):718–728. doi: 10.1515/znb-1970-0714. [DOI] [PubMed] [Google Scholar]
  3. Horn M. E., Sherrard J. H., Widholm J. M. Photoautotrophic growth of soybean cells in suspension culture: I. Establishment of photoautotrophic cultures. Plant Physiol. 1983 Jun;72(2):426–429. doi: 10.1104/pp.72.2.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Krivánková L., Dadák V. Semimicro extraction of ubiquinone and menaquinone from bacteria. Methods Enzymol. 1980;67:111–114. doi: 10.1016/s0076-6879(80)67016-5. [DOI] [PubMed] [Google Scholar]
  5. Norman M. A., Liebl R. A., Widholm J. M. Uptake and metabolism of clomazone in tolerant-soybean and susceptible-cotton photomixotrophic cell suspension cultures. Plant Physiol. 1990 Mar;92(3):777–784. doi: 10.1104/pp.92.3.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Rendina A. R., Felts J. M. Cyclohexanedione Herbicides Are Selective and Potent Inhibitors of Acetyl-CoA Carboxylase from Grasses. Plant Physiol. 1988 Apr;86(4):983–986. doi: 10.1104/pp.86.4.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rogers S. M., Ogren W. L., Widholm J. M. Photosynthetic characteristics of a photoautotrophic cell suspension culture of soybean. Plant Physiol. 1987 Aug;84(4):1451–1456. doi: 10.1104/pp.84.4.1451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Secor J., Cséke C. Inhibition of Acetyl-CoA Carboxylase Activity by Haloxyfop and Tralkoxydim. Plant Physiol. 1988 Jan;86(1):10–12. doi: 10.1104/pp.86.1.10. [DOI] [PMC free article] [PubMed] [Google Scholar]

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